C(-106)T polymorphism of the aldose reductase gene and the progression rate of diabetic retinopathy
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Date
2006
Journal Title
Journal ISSN
Volume Title
Publisher
ELSEVIER IRELAND LTD
Abstract
Purpose: To study the C(-106)T polymorphism in the promoter of the aldose reductase (ALR2) gene: (a) its local prevalence and (b) its modulation of the susceptibility for developing retinopathy.
Methods: DNAs of 96 control subjects and 53 long-standing (duration 17.9 +/- 5.4 years) type-2 diabetic patients were analyzed by PCR-RFLP with BfaI enzyme. Retinopathy was graded with 2-eye, 7-field fundus color photography. The IMF-HbA1c was the arithmetic mean of all HbA1c's of each patient.
Results: The genotypes in the controls were CC = 57 (59.4%), CT = 32 (33.3%) and TT = 7 (7.3%), with Hardy-Weinberg chi(2) = 0.793 (p > 0.50). Among 53 diabetics, CC = 24 (45.3%), CT = 26 (49.0%) and TT = 3 (5.7%). The correlation between IMF-HbA1c and retinopathy progression rate was significant on CC (r = 0.6102, p = 0.0072) but not in CT + TT genotypes (r = 0.26, p = 0.1811).
Conclusions: In Chilean adults, the frequency of the C(-106)T polymorphism of the ALR2 gene was similar to that reported by others. Type-2 diabetics with the CC genotype were more susceptible for developing retinopathy as a result of chronic hyperglycemia than those with the CT or TT genotype. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
Methods: DNAs of 96 control subjects and 53 long-standing (duration 17.9 +/- 5.4 years) type-2 diabetic patients were analyzed by PCR-RFLP with BfaI enzyme. Retinopathy was graded with 2-eye, 7-field fundus color photography. The IMF-HbA1c was the arithmetic mean of all HbA1c's of each patient.
Results: The genotypes in the controls were CC = 57 (59.4%), CT = 32 (33.3%) and TT = 7 (7.3%), with Hardy-Weinberg chi(2) = 0.793 (p > 0.50). Among 53 diabetics, CC = 24 (45.3%), CT = 26 (49.0%) and TT = 3 (5.7%). The correlation between IMF-HbA1c and retinopathy progression rate was significant on CC (r = 0.6102, p = 0.0072) but not in CT + TT genotypes (r = 0.26, p = 0.1811).
Conclusions: In Chilean adults, the frequency of the C(-106)T polymorphism of the ALR2 gene was similar to that reported by others. Type-2 diabetics with the CC genotype were more susceptible for developing retinopathy as a result of chronic hyperglycemia than those with the CT or TT genotype. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
Description
Keywords
aldose reductase, polymorphism, diabetes, retinopathy, MICROVASCULAR COMPLICATIONS, JAPANESE PATIENTS, MELLITUS, SUSCEPTIBILITY, NEPHROPATHY, REGION, NIDDM, RISK