Cellular immune responses in amniotic fluid of women with preterm prelabor rupture of membranes
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
WALTER DE GRUYTER GMBH
Abstract
Background: Preterm birth is the leading cause of perinatal morbidity and mortality. Preterm prelabor rupture of membranes (pPROM) occurs in 30% of preterm births; thus, this complication is a major contributor to maternal and neonatal morbidity. However, the cellular immune responses in amniotic fluid of women with pPROM have not been investigated.
Methods: Amniotic fluid samples were obtained from women with pPROM and a positive (n = 7) or negative (n =10) microbiological culture. Flow cytometry was performed to evaluate the phenotype and number of amniotic fluid leukocytes. The correlation between amniotic fluid immune cells and an interleukin-6 (IL-6) concentration or a white blood cell (WBC) count in amniotic fluid was calculated.
Results: Women with pPROM and a positive amniotic fluid culture had (1) a greater number of total leukocytes in amniotic fluid, including neutrophils and monocytes/macrophages and (2) an increased number of total T cells in amniotic fluid, namely CD4+ T cells and CD8+ T cells, but not B cells. The numbers of neutrophils and monocytes/macrophages were positively correlated with IL -6 concentrations and WBC counts in amniotic fluid of women with pPROM.
Conclusion: Women with pPROM and a positive amniotic fluid culture exhibit a more severe cellular immune response than those with a negative culture, which is associated with well-known markers of intra-amniotic inflammation.
Methods: Amniotic fluid samples were obtained from women with pPROM and a positive (n = 7) or negative (n =10) microbiological culture. Flow cytometry was performed to evaluate the phenotype and number of amniotic fluid leukocytes. The correlation between amniotic fluid immune cells and an interleukin-6 (IL-6) concentration or a white blood cell (WBC) count in amniotic fluid was calculated.
Results: Women with pPROM and a positive amniotic fluid culture had (1) a greater number of total leukocytes in amniotic fluid, including neutrophils and monocytes/macrophages and (2) an increased number of total T cells in amniotic fluid, namely CD4+ T cells and CD8+ T cells, but not B cells. The numbers of neutrophils and monocytes/macrophages were positively correlated with IL -6 concentrations and WBC counts in amniotic fluid of women with pPROM.
Conclusion: Women with pPROM and a positive amniotic fluid culture exhibit a more severe cellular immune response than those with a negative culture, which is associated with well-known markers of intra-amniotic inflammation.
Description
Keywords
acute chorioamnionitis, clinical chorioamnionitis, fetal inflammatory response, funisitis, innate immune cells, interleukin-6, labor, microbial invasion of the amniotic cavity, pregnancy, prematurity, preterm labor, MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA, ACTIVATING PEPTIDE-1 INTERLEUKIN-8, TUMOR-NECROSIS-FACTOR, PREMATURE RUPTURE, FETAL MEMBRANES, INTRAAMNIOTIC INFLAMMATION, MICROBIAL INVASION, INTRAUTERINE INFECTION, HUMAN PARTURITION, GRAM STAIN