Bacterial identification based on protein mass spectrometry: A new insight at the microbiology of the 21st century

Simple item page
dc.contributor.authorGarcia, Patricia
dc.contributor.authorAllende, Fidel
dc.contributor.authorLegarraga, Paulette
dc.contributor.authorHuilcaman, Marcos
dc.contributor.authorSolari, Sandra
dc.date.accessioned2024-01-10T13:46:18Z
dc.date.available2024-01-10T13:46:18Z
dc.date.issued2012
dc.description.abstractBacterial identification is important for the proper treatment of infected patients hospitalized with serious infections especially in critical care units. Identification by conventional methods used in microbiology laboratories takes at least 16 hours since a culture is positive. The introduction of mass spectrometry, specifically MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometer) in the microbiology laboratory could mean a radical change in the identification accuracy, turn around time (6 minutes per bacteria) and cost (about 5 times cheaper than conventional identification). Since its introduction in clinical microbiology laboratories in 2008, many reports about its usefulness in identifying microorganisms from colonies, as well as directly from positive blood cultures and urine samples have been published. This review describes MALDI-TOF MS methodology, its identification performance for bacteria (aerobic and anaerobic), mycobacterium and yeasts, its future applications in microbiology and its main disadvantages.
dc.fechaingreso.objetodigital2024-05-30
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.4067/S0716-10182012000300003
dc.identifier.eissn0717-6341
dc.identifier.issn0716-1018
dc.identifier.pubmedidMEDLINE:23096465
dc.identifier.urihttps://doi.org/10.4067/S0716-10182012000300003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79146
dc.identifier.wosidWOS:000305923600003
dc.information.autorucMedicina; Legarraga P;S/I;3210
dc.information.autorucMedicina;Allende F ;S/I;1004550
dc.information.autorucMedicina;Garcia P ;S/I;73909
dc.information.autorucMedicina;Huilcaman M ;S/I;199954
dc.information.autorucMedicina;Solari S ;S/I;1871
dc.issue.numero3
dc.language.isoes
dc.nota.accesoContenido completo
dc.pagina.final272
dc.pagina.inicio263
dc.publisherSOC CHILENA INFECTOLOGIA
dc.revistaREVISTA CHILENA DE INFECTOLOGIA
dc.rightsacceso abierto
dc.subjectBacterial identification
dc.subjectmass spectrometry
dc.subjectDESORPTION IONIZATION-TIME
dc.subjectLASER-DESORPTION/IONIZATION-TIME
dc.subjectBLOOD-STREAM INFECTIONS
dc.subjectSPECIES IDENTIFICATION
dc.subjectRAPID IDENTIFICATION
dc.subjectANTIMICROBIAL THERAPY
dc.subjectCULTURE-MEDIA
dc.subjectROUTINE
dc.subjectYEAST
dc.subjectMICROORGANISMS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBacterial identification based on protein mass spectrometry: A new insight at the microbiology of the 21st century
dc.typeartículo
dc.volumen29
sipa.codpersvinculados3210
sipa.codpersvinculados1004550
sipa.codpersvinculados73909
sipa.codpersvinculados199954
sipa.codpersvinculados1871
sipa.indexWOS
sipa.indexScielo
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Bacterial identification based on protein mass spectrometry; A new insight at the microbiology of the 21st century.pdf
Size:
3.83 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas