Bacterial identification based on protein mass spectrometry: A new insight at the microbiology of the 21st century
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
SOC CHILENA INFECTOLOGIA
Abstract
Bacterial identification is important for the proper treatment of infected patients hospitalized with serious infections especially in critical care units. Identification by conventional methods used in microbiology laboratories takes at least 16 hours since a culture is positive. The introduction of mass spectrometry, specifically MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometer) in the microbiology laboratory could mean a radical change in the identification accuracy, turn around time (6 minutes per bacteria) and cost (about 5 times cheaper than conventional identification). Since its introduction in clinical microbiology laboratories in 2008, many reports about its usefulness in identifying microorganisms from colonies, as well as directly from positive blood cultures and urine samples have been published. This review describes MALDI-TOF MS methodology, its identification performance for bacteria (aerobic and anaerobic), mycobacterium and yeasts, its future applications in microbiology and its main disadvantages.
Description
Keywords
Bacterial identification, mass spectrometry, DESORPTION IONIZATION-TIME, LASER-DESORPTION/IONIZATION-TIME, BLOOD-STREAM INFECTIONS, SPECIES IDENTIFICATION, RAPID IDENTIFICATION, ANTIMICROBIAL THERAPY, CULTURE-MEDIA, ROUTINE, YEAST, MICROORGANISMS