Replication kinetics of novel swine influenza A viruses: an approach to vaccine production

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dc.contributor.authorTapia, Rodrigo
dc.contributor.authorMedina, Rafael
dc.contributor.authorNeira, Victor
dc.date.accessioned2025-01-20T16:12:14Z
dc.date.available2025-01-20T16:12:14Z
dc.date.issued2024
dc.description.abstractNovel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step in vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers of >= 128 HAU/50 mu L with an MOI of 0.00001 at 60 h post-infection. These data may be useful in vaccine- producing laboratories.
dc.fuente.origenWOS
dc.identifier.doi10.4206/ajvs.562.01
dc.identifier.eissn0719-8132
dc.identifier.issn0719-8000
dc.identifier.urihttps://doi.org/10.4206/ajvs.562.01
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90321
dc.identifier.wosidWOS:001276409700004
dc.issue.numero2
dc.language.isoen
dc.pagina.final90
dc.pagina.inicio85
dc.revistaAustral journal of veterinary sciences
dc.rightsacceso restringido
dc.subjectInfluenza A virus
dc.subjectMDCK cells
dc.subjectVero cells
dc.subjectantigen
dc.subjectvaccine
dc.subjectswine
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleReplication kinetics of novel swine influenza A viruses: an approach to vaccine production
dc.typeartículo
dc.volumen56
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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