Replication kinetics of novel swine influenza A viruses: an approach to vaccine production
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Date
2024
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Abstract
Novel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step in vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers of >= 128 HAU/50 mu L with an MOI of 0.00001 at 60 h post-infection. These data may be useful in vaccine- producing laboratories.
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Influenza A virus, MDCK cells, Vero cells, antigen, vaccine, swine