3.18 Tesis doctorado

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A framework to analyze institutional systems for managing and governing water resources
(2023) Blanco, Elisa; Donoso H., Guillermo; Pontificia Universidad Católica de Chile. Facultad de Agronomía y Sistemas Naturales
Garantizar el acceso al agua implica más que una cuestión de disponibilidad, sino también una cuestión de gestión y gobernanza. Si bien algunos sistemas hídricos han demostrado éxito en la gestión eficiente de sus escasos recursos, muchos enfrentan desafíos cuando se trata de organizar y coordinar la asignación y gestión sostenible de sus recursos. Para comprender los sistemas de recursos naturales, se han desarrollado los Principios de Diseño de Bienes Comunes, los Sistemas Socio-ecológicos (SES, por sus siglas en inglés) y el marco de Análisis y Desarrollo Institucional (Institutional Analysis and Development framework, IAD), todos ellos arraigados en teorías de acción colectiva. Estas teorías se han empleado para comprender las formas en que operan los sistemas institucionales complejos y los, a menudo, intrincados procesos de toma de decisiones que tienen lugar dentro de ellos. Al mismo tiempo, para establecer reglas y comprender los roles dentro de estos sistemas institucionales, se ha desarrollado la teoría del diseño de mecanismos. Estas teorías aún no se han adaptado completamente para incorporar las complejidades y la fragmentación inherentes a los marcos institucionales del agua. Por lo tanto, la presente tesis pretende contribuir al avance del conocimiento y la comprensión sobre la gestión de los sistemas socio-ecológicos hídricos. Aspira a comprender, desentrañar, analizar y aplicar estas teorías, hacia el análisis de los sistemas hídricos. Con ello, el objetivo es desarrollar un marco analítico que pueda estudiar y comprender mejor los sistemas socio-ecológicos del agua para mejorar sus instituciones y gobernanza. Al hacerlo, este trabajo busca ofrecer orientación a una audiencia amplia, incluidos usuarios del agua, investigadores, formuladores de políticas y autoridades responsables de diseñar políticas y proteger la sostenibilidad de los recursos hídricos.
Ascophyllum nodosum-derived biostimulants modulate plant development through the regulation of hormones signaling
(2023) Pozo Valdés, Josefa del; O'Brien, José; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
In agriculture, biostimulants derived from Ascophyllum nodosum extracts (ANE) have been widely used as growth stimulants and for protecting crops against biotic and abiotic stresses. Despite their widespread use, we have limited knowledge about how these biostimulants work and their role in influencing phytohormones throughout the plant. To shed light on this, we conducted a study using two commercial ANE biostimulants, Exelgrow (EG) and Exelmax (EM), which are produced through different methods. The main objective was to characterize and compare the effects of these biostimulants on the phenotype and hormonal signaling in Arabidopsis thaliana. Our findings reveal that EG and EM exhibit different biostimulant activity in Arabidopsis. Furthermore, EG promotes cytokinin (CK) signaling and suppresses auxin (AUX) signaling in the root meristem, while EM stimulates AUX and inhibits CK signaling. The transcriptomic analysis provided insights into their mechanism of action, revealing a priming effect against stress. EG interacts mainly with salicylic acid (SA) and jasmonate pathways, collectively regulating the plant's response to various biotic stresses. On the other hand, EM interacts with the brassinosteroids (BR) pathway, regulating ROS signals and defense against nematodes and pathogens. Finally, the transcriptomic analysis suggest that EM could be indirectly regulating AUX through the downregulation of BR signaling.
Characterization of the primocane flowering trait in the red raspberry (Rubus idaeus L.) and the effect of low temperature
(2018) Contreras Moya, Elida; Gambardella C., Marina; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Characterization of viral agents present in tomato crops in Arica and Parinacota Region of Northern Chile
(2019) Rojas Bertini, Claudia Andrea; Rosales V., Marlene; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Copper-litter-soil interaction assessment in fruit tree productive systems
(2021) Schoffer Navarro, Jorge Tomás; Ginocchio Cea, Rosanna; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Las altas concentraciones de cobre en el suelo pueden provocar efectos adversos en los sistemas naturales y agrícolas. En este contexto, los plaguicidas a base de cobre se han utilizado para el control de enfermedades microbianas en los sistemas productivos de árboles frutales durante más de 200 años. Por lo tanto, el Cu se puede acumular en suelos agrícolas, afectando la microbiota del suelo y la degradación de la hojarasca. En este sentido, la hojarasca de árboles frutales puede constituir una barrera contra la incorporación de plaguicidas que contienen Cu en el suelo. El objetivo de este trabajo fue evaluar el efecto protector de la hojarasca contra la incorporación de Cu en el suelo, en términos de su relación lignina/N, en sistemas frutales en el centro de Chile. Los resultados confirman que la hojarasca ejerce un papel protector contra la incorporación de pesticidas que contienen Cu en el suelo, ya que el Cu se acumuló hasta 13 veces más en la hojarasca que en el suelo. Sin embargo, no fue posible discriminar si este papel protector afecta favorablemente la actividad microbiana del suelo. La función protectora no dependió de la relación lignina/N de la hojarasca como indicador de la capacidad de adsorción de Cu de la hojarasca y su degradación. Se deben realizar más estudios para evaluar si este papel protector de la hojarasca se manifiesta como una menor toxicidad de Cu en un bioindicador más adecuado, como las lombrices.
Development of predictive and detection models for internal browning, watercore and bitter pit in apples using Vis-NIR spectrometry.
(2019) Mogollón Lancheros, Miguel René; Zoffoli, Juan Pablo; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Development of Run1Ren1 grapevine plants (Vitis vinifera) resistant to Erysiphe necator (powdery mildew)
(2022) Sosa Zúñiga, Viviana; Arce Johnson, Jorge Patricio; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Eco-morphological effects of large wood in mountain basins, under different level of forest management
(2018) Ugalde Pascual, Fernando Javier; Mao, Luca; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Economic modelling of water storage, irrigation and crop choice under water scarcity
(2023) Strappa García de la Huerta, Valentina; Melo Contreras, Óscar; Pontificia Universidad Católica de Chile. Facultad de Agronomía y Sistemas Naturales
In the context of climate change's impact on water resources, this thesis investigates three adaptive strategies for water use in agriculture: - Changing the irrigation policy: Examining farmers' responses to water availability uncertainty, especially for permanent crops, the study employs a dynamic economic model considering intertemporal effects. The results emphasize the tradeoff between water dose and crop area under water restrictions, crucial for optimal irrigation decisions.- Water storage policies: Addressing the need for efficient water management, the research focus on dam operating rules. Integrating rational expectations, the study develops optimal storage policies based on expected future water resources. Findings underscore the importance of bounded capacity in mitigating the effects of decreased mean water inflows.- Changing the crop mix: Exploring water scarcity's impact on crop choice in low- to medium-income nations, the study proposes an economic model where water, not land, is the primary decision variable for farmers. The research employs a multinomial logistic structure to analyze water allocations for crop choices in Peru.This interdisciplinary research integrates dynamic economic modeling to provide insights into optimal irrigation, water storage, and crop choice strategies, contributing to understanding adaptive responses amid changing climatic conditions and resource pressures.
Efecto del fotoperiodo sobre la estacionalidad reproductiva en guanacos (Lama guanicoe) hembras
(2019) Correa Estrada, Lina María; Riveros Fernández, José Luis; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Effect of olive oil and hydrogenated vegetable oil supplementation on rumen bacterial composition in dairy cows
(2019) Cancino Padilla, Nathaly; Vargas Bello, Einar; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Effect of saline irrigation on the carotenoids biosynthesis and fruit development of Solanum lycopersicum cv. Micro Tom.
(2019) Leiva Ampuero, Andrés; Vega C., Andrea; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Salinity is an environmental factor that limits plants growth and crop productivity, affecting biochemical and physiological mechanisms, as well as morphology in diverse plants. Decreasing in the photosynthetic capacity triggers important changes in plant development. It has been described that saline stress induce lipids, proteins and nucleic acids oxidative damage. To survive to these negative conditions, plants synthesize antioxidant molecules and detoxifying enzymes, in order to protect plant tissues against propagation of oxidative and cytotoxics species. In this context, Lycopene and ß-carotene have been correlated with antioxidant activities in several fruits. In a plant with moderate tolerance to salinity, such as tomato, high levels of NaCl decrease fruit size, number of leaves and stomatal density, reduce crop yield and negatively affect the carotenoids concentration. Interestingly, tomato plants exposed to low salinity significantly modifies the photosynthetic capacity, enhances carotenoid fruit accumulation and could improve tomato taste by increasing sugar and organic acids content. In this work, the effect of saline irrigation on the carotenoids biosynthesis and fruit development of Solanum lycopersicum cv. Micro Tom was studied. The results showed that after 8 weeks of irrigation with a 80mM to 160mM NaCl solutions negatively modified photosynthetic capacity, in terms of net photosynthesis, stomatic conductivity, photochemical efficiency, electron transport rate and non photochemical quenching. Also, after 14 weeks of a 40 to 160 mM salinity treatments, tomato fruits showed an early coloration, increasing the solid soluble content in comparison with the control. However, a reduction in fruit caliber and fresh weight was observed from 80 mM NaCl onwards. Finally, salinity treatments differentially changed expression of carotenoid biosynthetic genes and increased accumulation of several carotenoids under 120 to 160mM. Altogether, these results suggest that long term salt irrigation correlates with earliness in tomato, a major breeding characteristic in crop plants.Salinity is an environmental factor that limits plants growth and crop productivity, affecting biochemical and physiological mechanisms, as well as morphology in diverse plants. Decreasing in the photosynthetic capacity triggers important changes in plant development. It has been described that saline stress induce lipids, proteins and nucleic acids oxidative damage. To survive to these negative conditions, plants synthesize antioxidant molecules and detoxifying enzymes, in order to protect plant tissues against propagation of oxidative and cytotoxics species. In this context, Lycopene and ß-carotene have been correlated with antioxidant activities in several fruits. In a plant with moderate tolerance to salinity, such as tomato, high levels of NaCl decrease fruit size, number of leaves and stomatal density, reduce crop yield and negatively affect the carotenoids concentration. Interestingly, tomato plants exposed to low salinity significantly modifies the photosynthetic capacity, enhances carotenoid fruit accumulation and could improve tomato taste by increasing sugar and organic acids content. In this work, the effect of saline irrigation on the carotenoids biosynthesis and fruit development of Solanum lycopersicum cv. Micro Tom was studied. The results showed that after 8 weeks of irrigation with a 80mM to 160mM NaCl solutions negatively modified photosynthetic capacity, in terms of net photosynthesis, stomatic conductivity, photochemical efficiency, electron transport rate and non photochemical quenching. Also, after 14 weeks of a 40 to 160 mM salinity treatments, tomato fruits showed an early coloration, increasing the solid soluble content in comparison with the control. However, a reduction in fruit caliber and fresh weight was observed from 80 mM NaCl onwards. Finally, salinity treatments differentially changed expression of carotenoid biosynthetic genes and increased accumulation of several carotenoids under 120 to 160mM. Altogether, these results suggest that long term salt irrigation correlates with earliness in tomato, a major breeding characteristic in crop plants.Salinity is an environmental factor that limits plants growth and crop productivity, affecting biochemical and physiological mechanisms, as well as morphology in diverse plants. Decreasing in the photosynthetic capacity triggers important changes in plant development. It has been described that saline stress induce lipids, proteins and nucleic acids oxidative damage. To survive to these negative conditions, plants synthesize antioxidant molecules and detoxifying enzymes, in order to protect plant tissues against propagation of oxidative and cytotoxics species. In this context, Lycopene and ß-carotene have been correlated with antioxidant activities in several fruits. In a plant with moderate tolerance to salinity, such as tomato, high levels of NaCl decrease fruit size, number of leaves and stomatal density, reduce crop yield and negatively affect the carotenoids concentration. Interestingly, tomato plants exposed to low salinity significantly modifies the photosynthetic capacity, enhances carotenoid fruit accumulation and could improve tomato taste by increasing sugar and organic acids content. In this work, the effect of saline irrigation on the carotenoids biosynthesis and fruit development of Solanum lycopersicum cv. Micro Tom was studied. The results showed that after 8 weeks of irrigation with a 80mM to 160mM NaCl solutions negatively modified photosynthetic capacity, in terms of net photosynthesis, stomatic conductivity, photochemical efficiency, electron transport rate and non photochemical quenching. Also, after 14 weeks of a 40 to 160 mM salinity treatments, tomato fruits showed an early coloration, increasing the solid soluble content in comparison with the control. However, a reduction in fruit caliber and fresh weight was observed from 80 mM NaCl onwards. Finally, salinity treatments differentially changed expression of carotenoid biosynthetic genes and increased accumulation of several carotenoids under 120 to 160mM. Altogether, these results suggest that long term salt irrigation correlates with earliness in tomato, a major breeding characteristic in crop plants.Salinity is an environmental factor that limits plants growth and crop productivity, affecting biochemical and physiological mechanisms, as well as morphology in diverse plants. Decreasing in the photosynthetic capacity triggers important changes in plant development. It has been described that saline stress induce lipids, proteins and nucleic acids oxidative damage. To survive to these negative conditions, plants synthesize antioxidant molecules and detoxifying enzymes, in order to protect plant tissues against propagation of oxidative and cytotoxics species. In this context, Lycopene and ß-carotene have been correlated with antioxidant activities in several fruits. In a plant with moderate tolerance to salinity, such as tomato, high levels of NaCl decrease fruit size, number of leaves and stomatal density, reduce crop yield and negatively affect the carotenoids concentration. Interestingly, tomato plants exposed to low salinity significantly modifies the photosynthetic capacity, enhances carotenoid fruit accumulation and could improve tomato taste by increasing sugar and organic acids content. In this work, the effect of saline irrigation on the carotenoids biosynthesis and fruit development of Solanum lycopersicum cv. Micro Tom was studied. The results showed that after 8 weeks of irrigation with a 80mM to 160mM NaCl solutions negatively modified photosynthetic capacity, in terms of net photosynthesis, stomatic conductivity, photochemical efficiency, electron transport rate and non photochemical quenching. Also, after 14 weeks of a 40 to 160 mM salinity treatments, tomato fruits showed an early coloration, increasing the solid soluble content in comparison with the control. However, a reduction in fruit caliber and fresh weight was observed from 80 mM NaCl onwards. Finally, salinity treatments differentially changed expression of carotenoid biosynthetic genes and increased accumulation of several carotenoids under 120 to 160mM. Altogether, these results suggest that long term salt irrigation correlates with earliness in tomato, a major breeding characteristic in crop plants.Salinity is an environmental factor that limits plants growth and crop productivity, affecting biochemical and physiological mechanisms, as well as morphology in diverse plants. Decreasing in the photosynthetic capacity triggers important changes in plant development. It has been described that saline stress induce lipids, proteins and nucleic acids oxidative damage. To survive to these negative conditions, plants synthesize antioxidant molecules and detoxifying enzymes, in order to protect plant tissues against propagation of oxidative and cytotoxics species. In this context, Lycopene and ß-carotene have been correlated with antioxidant activities in several fruits. In a plant with moderate tolerance to salinity, such as tomato, high levels of NaCl decrease fruit size, number of leaves and stomatal density, reduce crop yield and negatively affect the carotenoids concentration. Interestingly, tomato plants exposed to low salinity significantly modifies the photosynthetic capacity, enhances carotenoid fruit accumulation and could improve tomato taste by increasing sugar and organic acids content. In this work, the effect of saline irrigation on the carotenoids biosynthesis and fruit development of Solanum lycopersicum cv. Micro Tom was studied. The results showed that after 8 weeks of irrigation with a 80mM to 160mM NaCl solutions negatively modified photosynthetic capacity, in terms of net photosynthesis, stomatic conductivity, photochemical efficiency, electron transport rate and non photochemical quenching. Also, after 14 weeks of a 40 to 160 mM salinity treatments, tomato fruits showed an early coloration, increasing the solid soluble content in comparison with the control. However, a reduction in fruit caliber and fresh weight was observed from 80 mM NaCl onwards. Finally, salinity treatments differentially changed expression of carotenoid biosynthetic genes and increased accumulation of several carotenoids under 120 to 160mM. Altogether, these results suggest that long term salt irrigation correlates with earliness in tomato, a major breeding characteristic in crop plants.
Ethylene and Nitrogen crosstalk in Solanum lycopersicum defense against Botrytis cinerea infection
(2023) Huidobro Marín, Camila; Arce Johnson, Jorge Patricio; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Durante la respuesta de defensa a Botrytis cinerea, Solanum lycopersicum presenta una menor susceptibilidad mientras exista una mayor biodisponibilidad de nitrógeno. El mecanismo por el que esto ocurre no se encuentra dilucidado. Cabe destacar que en otras especies como Arabidopsis thaliana, se da el caso contrario. En este trabajo, se estudió una posible diafonía entre etileno y nitrógeno mediando la defensa en plantas de tomate infectadas con botrytis. Para abordar este tema, se realizaron tratamientos con etileno exógeno y un inhibidor de esta hormona en plantas crecidas en concentraciones contrastantes de nitrógeno e infectadas con botrytis. Se evaluó el tamaño de lesión durante la progresión de la enfermedad, encontrando que las lesiones presentan diámetros significativamente menores en los grupos tratados con etileno y altas concentraciones de nitrógeno disponible. Se identificaron genes de etileno, defensa y nitrógeno que responden diferencialmente a la combinación de la etileno, nitrógeno y tiempo de infección. Paralelamente, se realizó una comparación de los transcriptomas de tomate y A. thaliana, identificando diferencias sutiles en el comportamiento transcriptómico de ambas especies asociadas a la biodisponibilidad de nitrógeno. En conjunto, estos resultados indican una diafonía entre etileno y nitrógeno dependiente del tiempo de infección durante el desarrollo de la enfermedad causada por botrytis en plantas de tomate.
Evaluation of growing and photosynthetic parameters in response to simple and mixed infections with GRSPaV in plants of Vitis vinifera L., "Cabernet Sauvignon" cultivar and study of new strategies of GRSPaV elimination
(2019) Tobar Urzúa, Mariola; Rosales V., Marlene; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Genetic and transcriptomic studies of testa color of Chilean landraces of common bean (Phaseolus vulgaris L.)
(2023) Rodríguez Alvarez, Yohaily; Schwember Neira, Andrés R.; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
El color de la testa de la semilla es una característica influenciada por la expresión de genes responsables de la producción y acumulación de compuestos fenólicos. Este rasgo resulta relevante en la capacidad comercial del poroto común y la calidad nutricional de su semilla. En este proyecto de tesis doctoral, se realizó un estudio genético y transcriptómico del color de la testa y la respuesta a déficit de riego utilizando variedades chilenas de poroto común. Consideradas muy probablemente el reservorio más antiguo del pool andino de poroto común y un recurso genético crucial para fines evolutivos, de mejoramiento y alimentarios. Se realizó un análisis de los compuestos fenólicos asociados al color de la testa en tres etapas reproductivas diferentes del desarrollo fenológico del cultivo, bajo el efecto de tratamientos hídricos contrastantes. Se realizó un análisis RNAseq para identificar genes candidatos responsables de la producción y acumulación de compuestos fenólicos en la testa, y se cuantificó su expresión. Se encontró que durante los estados fenológicos R7 y R8 de la variedad 'Peumo', se acumula un mayor contenido de taninos condensados, incluyendo catequina y epicatequina, en la testa del poroto en comparación con la variedad 'Tórtola'. Se identificó cuatro genes candidatos (Phvul.007G206000, Phvul.002G079300, Phvul.010G090300 y Phvul.009G040700) implicados en la producción y acumulación de taninos condensados, en este tejido bajo riego deficitario. Se realizó un análisis combinado de datos metabólicos y transcriptómicos un gen candidato (Phvul.002G079300) con regulación a la baja del flujo metabólico para reducir la producción y el transporte de monómeros de proantocianidinas en 'Tórtola' en comparación con 'Peumo'. Por primera vez, se reportan hallazgos novedosos para futuros estudios moleculares y bioquímicos de la coloración de la testa del poroto chileno que son importantes para los productores de poroto, la industria alimentaria y los consumidores.
Implementation of a Decision Support Rule for fungicides application against Botrytis cinerea in organic vineyards in Chile
(2023) Herrera Défaz, Mario Andrés; Valdés Gómez, Héctor; Pontificia Universidad Católica de Chile. Facultad de Agronomía y Sistemas Naturales
Este trabajo se enfocó en el desarrollo e implementación de una regla de decisión para el manejo de Botrytis cinerea, un hongo patógeno que afecta a los viñedos. El objetivo principal de esta investigación fue proporcionar una herramienta práctica y efectiva para los productores de uva orgánica, permitiéndoles optimizar el uso de biofungicidas y reducir los riesgos asociados con la enfermedad. La investigación se enfocó en la necesidad de encontrar soluciones que sean compatibles con los principios de la agricultura orgánica, evitando el uso de productos químicos sintéticos. En este sentido, se evaluaron diferentes enfoques, incluyendo el uso de biofungicidas comerciales y una nueva molécula desarrollada a partir de quitosano y riboflavina. La metodología utilizada incluyó la recopilación de datos sobre la incidencia de Botrytis cinerea en viñedos orgánicos, así como pruebas de laboratorio para evaluar la eficacia de los diferentes tratamientos biofungicidas. Los resultados de la investigación demostraron la viabilidad y eficacia de la regla de decisión propuesta para el manejo de Botrytis cinerea en viñedos orgánicos usando indicadores clave en el desarrollo fenológico del cultivo. Esta regla de decisión se basa en un conjunto de criterios que tienen en cuenta factores como el estado fenológico de la vid, las condiciones climáticas locales (temperatura y humedad relativa). Con relación a los tratamientos evaluados, se encontró que tanto algunos de los biofungicidas comerciales, y la nueva molécula basada en quitosano y riboflavina mostraron resultados prometedores en la reducción de la incidencia del patógeno. Estos hallazgos sugieren que existe un potencial considerable para el desarrollo de alternativas sustentables y efectivas para el manejo de botritis en viñedos orgánicos.
Interaction and management of begomoviruses and whiteflies in weeds and horticulture crops in the State of São Paulo and survey of orthotospoviruses and associated thrips in the Arica y Parinacota Region - Chile
(2021) Gorayeb, Eduardo Silva; Rosales V., Marlene; Sakate, Renate Krause; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Con el fin de aportar información clave para el manejo de mosquitas blancas, trips, Orthotospovirus y begomovirus en cultivos de tomate (Solanum lycopersicum L.), pepino (Cucumis sativus L.) y pimiento (Capsicum spp.), Esta tesis contempla dos estudios que involucran las especies crípticas de B. tabaci MEAM1 y MED en Brasil, y dos estudios de orthotospovirus y trips en Chile. Se estudió Datura stramonium L. y Nicandra physaloides L. (Gaertn.) como huéspedes alternativos de mosquita blanca y del virus de la rugosa severa del tomate (ToSRV), mostrando N. physaloides como un gran huésped alternativo del virus cuando se asocia con MEAM1, mientras que D. stramonium contribuyó más con la reproducción de la mosquita blanca. Luego, se investigaron las causas relacionadas con las recientes infestaciones de MED en pepinos observadas en Brasil, utilizando cultivares comerciales de pepino, demostrando un mejor desempeño de MED en casi todos los cultivares comerciales evaluados y que el desempeño es un factor clave para el predominio de esta mosquita blanca en las plantas de pepino. Finalmente, se reportó por primera vez en Chile el nuevo orthotospovirus putativo Pepper necrotic spot virus, infectando frutos de pimiento morrón, y en ocurrencia conjunta con el TSWV en la región de Arica y Parinacota (Norte de Chile) asociado a F. occidentalis, donde cada vez más casos de la enfermedad conocida por “el Bronceado del tomate/pimiento) está aumentando.
Modeling farmer production and food-loss decisions: a quality-based approach
(2023) Nunes Jansen, Sarah; Foster Bonnette, William; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Multivariate standardized indices - application beyond drought detection for operational drought management in semi-arid regions in the Americas
(2018) Oertel, Melanie; Meza, Francisco Javier; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Physiological behavior of own rooted vine plants and rootstock effect on growth and PIP and TIP aquaporin leaf expression in Vitis vinifera L. (cv. Cabernet Sauvignon)
(2022) Galaz Seguel, Andrea Paz; Gambardella C., Marina; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Water scarcity is an increasingly important problem affecting the main wine-producing areas, and therefore, it is of great interest to study the physiological and molecular mechanisms that occur during periods of drought. Based on the stomatal behavior, two different strategies for facing water stress have been described: isohydric and anisohydric. Nevertheless, this classification is still not clear, and the effect of the rootstock on the whole plant response is still unknown. In this context, two assays were carried out, in both plants were subjected to water stress periods of 7 and 14 days and to a recovery period. In the first one, four Vitis spp. own-rooted genotypes were studied, two rootstocks (1103P and SO4) and two cultivars (Cabernet Sauvignon and Carménère). Stem water potential, stomatal conductance, and growth rate were evaluated. Near anisohydric behavior was observed in all genotypes studied. The second assay consisted of three scion/rootstock combinations, Cabernet Sauvignon grafted onto itself, 1103P and 101-14. Although no differences were observed in stomatal conductance and stem water potential, near isohydric behavior was observed for the homograft, and near anisohydric behavior was found for the combinations. During the recovery period, significant differences were detected in the leaf expression levels of aquaporin genes VvPIP2;1, VvPIP2;2, VvTIP1;1 and VvTIP2;1. Cabernet Sauvignon plants grafted over 1103P and 101-14- showed higher expression levels. C. Sauvignon combined with rootstocks of different genetic backgrounds, responded better to the water suspension treatment, and its recovery was faster, demonstrating the benefits of genotype interactions in these responses.

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