Comparative effect of fish oil feeding and other dietary fatty acids on plasma lipoproteins, biliary lipids, and hepatic expression of proteins involved in reverse cholesterol transport in the rat

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dc.contributor.authorMorgado, N
dc.contributor.authorRigotti, A
dc.contributor.authorValenzuela, A
dc.date.accessioned2024-01-10T12:04:42Z
dc.date.available2024-01-10T12:04:42Z
dc.date.issued2005
dc.description.abstractBackground: While elevated plasma high-density lipoprotein (HDL) levels has been associated to a reduction in cardiovascular risk, dietary fish oils rich in omega-3 polyunsaturated fatty acids (PUFAs) may protect against this disease. The protective effect of HDL is associated to its participation in the reverse cholesterol transport pathway. On the other hand, omega-3 PUFAs decrease plasma HDL levels compared to other fatty acids, which may suggest an effect on reverse cholesterol transport. Aim: In this work, the effect of dietary fish oil on the fatty acid composition of hepatic membranes, plasma lipoprotein cholesterol profile, biliary lipids, and the expression of proteins involved in reverse cholesterol transport, was compared to other dietary oils having a different degree of fatty acid unsaturation. Methods: Male rats were fed a semi synthetic diet containing fish oil ( omega -3), sunflower oil (omega-6), olive oil (omega-9) or coconut oil (saturated). Hepatic membrane fatty acid composition, plasma cholesterol levels, lipoprotein cholesterol profile, biliary lipids, hepatic mRNA levels for lecithin cholesterol acyltransferase, hepatic lipase, apo E, and apo A-I, and hepatic protein levels of the scavenger receptor class B type I, caveolin-1, and the ATP binding cassette transporter A1 were analyzed. Plasma apo A-I and apo E protein levels were also evaluated. Results: Compared to the other diets, omega - 3 PUFAs significantly changed omega-3/omega-6 fatty acid ratio of hepatic membranes, caused a reduction of plasma total and HDL cholesterol, and selectively increased biliary cholesterol secretion. No modifi cation in the expression levels of lecithin cholesterol acyltransferase, hepatic lipase, apo A-I and apo E mRNA was observed. Hepatic scavenger receptor class B type I, caveolin-1, and the ATP binding cassette transporter A1 protein levels were also not affected. Plasma apo A-I, but not apo E, was reduced. Conclusions: These results show that dietary omega-3 PUFAs reduce plasma HDL cholesterol and increase biliary cholesterol without concomitant modifi cations in the expression of key genes and proteins involved in reverse cholesterol transport. These fi ndings suggest that functional changes in the activity of these proteins as consequence of the incorporation of omega3 PUFAs into hepatic membranes and plasma lipoproteins may underlie the effect of fi sh oil feeding on plasma and hepatic cholesterol metabolism in the rat. Copyright (c) 2005 S. Karger AG, Basel.
dc.fechaingreso.objetodigital2024-05-16
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1159/000088935
dc.identifier.eissn1421-9697
dc.identifier.issn0250-6807
dc.identifier.pubmedidMEDLINE:16227687
dc.identifier.urihttps://doi.org/10.1159/000088935
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75860
dc.identifier.wosidWOS:000233904100007
dc.information.autorucMedicina;Rigotti A;S/I;68489
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final406
dc.pagina.inicio397
dc.publisherKARGER
dc.revistaANNALS OF NUTRITION AND METABOLISM
dc.rightsacceso restringido
dc.subjectreverse cholesterol transport
dc.subjectpolyunsaturated fatty acids
dc.subjecthigh density lipoprotein-cholesterol
dc.subjectSR-BI
dc.subjectlecithin : cholesterol acyltransferase
dc.subjectATP-binding cassette transporter A1
dc.subjectapolipoproteins
dc.subjectCORONARY-HEART-DISEASE
dc.subjectSCAVENGER RECEPTOR B1
dc.subjectSR-BI
dc.subjectDOCOSAHEXAENOIC ACID
dc.subjectHDL-CHOLESTEROL
dc.subjectLIVER
dc.subjectFLUIDITY
dc.subjectEFFLUX
dc.subjectACYLTRANSFERASE
dc.subjectSECRETION
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleComparative effect of fish oil feeding and other dietary fatty acids on plasma lipoproteins, biliary lipids, and hepatic expression of proteins involved in reverse cholesterol transport in the rat
dc.typeartículo
dc.volumen49
sipa.codpersvinculados68489
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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