The effect of proanthocyanidin-containing 10% phosphoric acid on bonding properties and MMP inhibition

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dc.contributor.authorHass, V.
dc.contributor.authorLuque Martínez, Íssis Virginia
dc.contributor.authorMuñoz Figueroa, Marcelo Alejandro
dc.contributor.authorReyes, M. F. G.
dc.contributor.authorAbuna, G.
dc.contributor.authorSinhoreti, M. A. C.
dc.contributor.authorLiu, A. Y.
dc.contributor.authorLoguercio, A. D.
dc.contributor.authorWang, Y.
dc.contributor.authorReis, A.
dc.date.accessioned2019-10-16T20:16:11Z
dc.date.available2019-10-16T20:16:11Z
dc.date.issued2016
dc.description.abstractObjectives. This study evaluated the effect of etching using 2% proanthocynidin-containing 10% phosphoric acid 2% PA/10% PhA vs. 35% phosphoric acid 35% PhA on immediate (IM) and 6-months (6 M) resin-enamel microshear bond strength (mu SBS), resin-dentin microtensile bond strength (mu TBS), nanoleakage (NL) and as well as in situ MMP inhibition potential. Methods. The dentin surface of human were exposed and then etched using 35% phosphoric acid for 15 s or 2% PA/10% phosphoric acid for 30 s. After rinsing with water, the dentin was bonded with Single Bond Plus (3 M ESPE) and composite build-ups were constructed, followed by polymerization. The teeth were sectioned and the bonds were testing for microtensile bond strength (mu TBS) and by SEM for NL analysis at IM and 6 M. For MMP activity, resin-dentin slices were prepared for in situ zymography, and analyzed under con focal microscopy. For mu SBS, others teeth had flattened enamel surfaces etched according the experimental groups and prepared to microshear procedure. The specimens were tested IM and after 6 M by microshear bond strength. The data were submitted to two-way repeated measures ANOVA and Tukey's test (alpha = 0.05). Results. Acid-etching using the 2% PA/10% phosphoric acid did not lower the mu TBS in IM (p > 0.05) compared to the control 35% phosphoric acid group. However, after 6 M, only the 2% PA/10% PhA etched dentin had remained stable the resin-dentin bond strength (p < 0.05). Bonds made with 35% PhA showed significant increase in NL% after 6 M (p < 0.05). Dentin bonds made with 2% PA/10% phosphoric acid showed no increase in NL% after 6 months. The MMP activity within the resin-dentin interface was almost completely reduced after 2% PA/10% PhA etching, while the 35% PhA exhibited intense MMP activity. For mu SBS, the type of etchant and the storage period did not affect the resin-enamel bond strengths (p > 0.05). Significance. Ten percent phosphoric acid containing 2% PA can produce stable resin-dentin and enamel-resin interfaces, without requiring additional steps in the bonding procedure. Future studies for longer evaluation time are required. (C) 2016 Published by Elsevier Ltd on behalf of Academy of Dental Materials. All rights reserved.
dc.format.extent8 páginas
dc.fuente.origenFacultad de Medicina
dc.identifier.doi10.1016/j.dental.2015.12.007
dc.identifier.issn0109-5641
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26571
dc.issue.numeroNo. 3
dc.language.isoen
dc.pagina.final475
dc.pagina.inicio468
dc.revistaDental Materialses_ES
dc.rightsacceso restringido
dc.subjectDentin collagenes_ES
dc.subjectAcid etchinges_ES
dc.subjectCrosslinking reagentses_ES
dc.subjectProanthocyanidinses_ES
dc.subjectDemineralized dentin layeres_ES
dc.subjectMMPses_ES
dc.subjectchlorhexidine-containing acides_ES
dc.subjectresin-dentin interfaceses_ES
dc.subjectcross-linkinges_ES
dc.subjecthybrid layeres_ES
dc.subjectin-vitroes_ES
dc.subjectrinse adhesiveses_ES
dc.subjectcured resines_ES
dc.subjectenameles_ES
dc.subjectstrengthes_ES
dc.subjectdurabilityes_ES
dc.subjectDentistry, Oral Surgery & Medicinees_ES
dc.subjectMaterials Sciencees_ES
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherDentinaes_ES
dc.subject.otherÁcidos fosfóricoses_ES
dc.subject.otherArándanos - Uso Terapéuticoes_ES
dc.subject.otherColágenoes_ES
dc.titleThe effect of proanthocyanidin-containing 10% phosphoric acid on bonding properties and MMP inhibitiones_ES
dc.typeartículo
dc.volumenVol. 32
sipa.codpersvinculados1020227
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