Differences between nuclear run-off and mRNA levels for multidrug resistance gene expression in the cephalocaudal axis of the mouse Intestine

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dc.contributor.authorChianale, J
dc.contributor.authorVollrath, V
dc.contributor.authorWielandt, AM
dc.contributor.authorMiranda, S
dc.contributor.authorGonzalez, R
dc.contributor.authorFresno, AM
dc.contributor.authorQuintana, C
dc.contributor.authorGonzalez, S
dc.contributor.authorAndrade, L
dc.contributor.authorGuzman, S
dc.date.accessioned2024-01-10T12:04:56Z
dc.date.available2024-01-10T12:04:56Z
dc.date.issued1995
dc.description.abstractP-glycoprotein is a multidrug transporter encoded by the mdr3 gene in the mouse intestinal epithelium. The aims of this study were to characterize the mdr3 gene expression in the cephalocaudal axis of the intestine in adult animals and during perinatal development, and to define the molecular mechanism responsible for the heterogeneous expression of the gene along the cephalocaudal axis. RNA extracted from stomach, duodenum, jejunum, ileum, cecum and colon was hybridized by slot blot and Northern blot using a mdr3 cDNA probe. The regulation of gene expression was investigated examining the rate of transcription by nuclear run-off analysis. Transport studies of rhodamine 123, a substrate of P-glycoprotein, were performed in everted jejunum and ileum. The level of mdr3 mRNA and P-glycoprotein found in ileum was 6-fold higher than the level found in duodenum. The regional pattern of mdr3 gene expression is established in the intestine of 10-day-old animals. Similar mdr3 hybridization signal in nuclear run-off assay was found in nuclei of enterocytes isolated from jejunum and ileum, suggesting that the heterogeneous expression of the mdr3 gene in the cephalocaudal axis of the small bowel may be predominantly regulated at the post-transcriptional level. Transport rate of rhodamine 123 from the serosal to mucosal side in everted ileum was higher than the rate of transport found in jejunum. These results indicate that enterocytes of the ileum may be more actively involved in the P-glycoprotein-mediated transport of xenobiotics into the intestinal lumen.
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/0167-4781(95)00179-4
dc.identifier.issn0167-4781
dc.identifier.pubmedidMEDLINE:8547326
dc.identifier.urihttps://doi.org/10.1016/0167-4781(95)00179-4
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75911
dc.identifier.wosidWOS:A1995TP08300021
dc.information.autorucMedicina;Chianale J;S/I;99780
dc.information.autorucMedicina;González S;S/I;99856
dc.information.autorucMedicina;Quintana C;S/I;102210
dc.issue.numero3
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final376
dc.pagina.inicio369
dc.publisherELSEVIER SCIENCE BV
dc.revistaBIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
dc.rightsregistro bibliográfico
dc.subjectintestinal differentiation
dc.subjectgene regulation
dc.subjectP-glycoprotein
dc.subjectmultidrug transporter
dc.subjectmdr gene
dc.subjectP-GLYCOPROTEIN
dc.subjectCELL-LINES
dc.subjectCANALICULAR MEMBRANE
dc.subjectTRANSGENIC MICE
dc.subjectMESSENGER-RNA
dc.subjectBRUSH-BORDER
dc.subjectRAT-LIVER
dc.subjectFAMILY
dc.subjectANTIBODIES
dc.subjectCLONING
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferences between nuclear run-off and mRNA levels for multidrug resistance gene expression in the cephalocaudal axis of the mouse Intestine
dc.typeartículo
dc.volumen1264
sipa.codpersvinculados99780
sipa.codpersvinculados99856
sipa.codpersvinculados102210
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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