Down-regulation of intestinal scavenger receptor class B, type I (SR-BI) expression in rodents under conditions of deficient bile delivery to the intestine

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dc.contributor.authorVoshol, PJ
dc.contributor.authorSchwarz, M
dc.contributor.authorRigotti, A
dc.contributor.authorKrieger, M
dc.contributor.authorGroen, AK
dc.contributor.authorKuipers, F
dc.date.accessioned2024-01-10T13:12:27Z
dc.date.available2024-01-10T13:12:27Z
dc.date.issued2001
dc.description.abstractScavenger receptor class B, type I (SR-BI) is expressed in the intestines of rodents and has been suggested to be involved in the absorption of dietary cholesterol. The aim of this study was to determine whether intestinal SR-BI expression is affected in animal models with altered bile delivery to the intestine and impaired cholesterol absorption. SR-BI protein and mRNA levels were determined in proximal and distal small intestine from control, bile-duct-ligated and bile-diverted rats and from control and bile-duct-ligated mice. Two genetically altered mouse models were studied: multidrug resistance-2 P-glycoprotein-deficient [Mdr2((-/-))] mice that produce phospholipid/cholesterol-free bile, and cholesterol 7 alpha -hydroxylase-deficient [Cyp7a((-/-))] mice, which exhibit qualitative and quantitative changes in the bile-salt pool. Cholesterol-absorption efficiency was quantified using a dual-isotope ratio method. SR-BI was present at the apical membrane of enterocytes in control rats and mice and was more abundant in proximal than in distal segments of the intestine. In bile-duct-ligated animals, levels of SR-BI protein were virtually absent and mRNA levels were decreased by approximate to 50 %. Bile-diverted rats, Mdr2((-/-)) mice and Cyp7a((-/-)) mice showed decreased levels of intestinal SR-BI protein while mRNA levels were unaffected. Cholesterol absorption was reduced by > 90% in bile-duct-ligated and bile-diverted animals and in Cyp7a((-/-)) mice, whereas Mdr2((-/-)) mice showed an approximate to 50% reduction. This study shows that SR-BI is expressed at the apical membrane of enterocytes of rats and mice; mainly in the upper intestine where cholesterol absorption is greatest, and indicates that bile components play a role in post-transcriptional regulation of SR-BI expression. Factors associated with cholestasis appear to be involved in transcriptional control of intestinal SR-BI expression. The role of SR-BI in the cholesterol-absorption process remains to be defined.
dc.description.funderNHLBI NIH HHS
dc.description.funderNIDDK NIH HHS
dc.description.funderNATIONAL HEART, LUNG, AND BLOOD INSTITUTE
dc.description.funderNATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
dc.fechaingreso.objetodigital2024-05-02
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1042/0264-6021:3560317
dc.identifier.eissn1470-8728
dc.identifier.issn0264-6021
dc.identifier.pubmedidMEDLINE:11368757
dc.identifier.urihttps://doi.org/10.1042/0264-6021:3560317
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78187
dc.identifier.wosidWOS:000169295800003
dc.information.autorucMedicina;Rigotti A;S/I;68489
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final325
dc.pagina.inicio317
dc.publisherPORTLAND PRESS LTD
dc.revistaBIOCHEMICAL JOURNAL
dc.rightsacceso restringido
dc.subjectabsorption
dc.subjectbile salts
dc.subjectcholesterol
dc.subjectknockout mouse
dc.subjectphospholipid
dc.subjectDENSITY-LIPOPROTEIN RECEPTOR
dc.subjectCHOLESTEROL 7-ALPHA-HYDROXYLASE GENE
dc.subjectPHYSICAL-CHEMICAL BEHAVIOR
dc.subjectPLASMA RATIO METHOD
dc.subjectADULT HUMAN-BEINGS
dc.subjectDIETARY-CHOLESTEROL
dc.subjectBILIARY CHOLESTEROL
dc.subjectAGGREGATION STATES
dc.subjectLINOLEIC-ACID
dc.subjectHDL RECEPTOR
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDown-regulation of intestinal scavenger receptor class B, type I (SR-BI) expression in rodents under conditions of deficient bile delivery to the intestine
dc.typeartículo
dc.volumen356
sipa.codpersvinculados68489
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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