Exo70 protects against memory and synaptic impairments following mild traumatic brain injury
Loading...
Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Mild traumatic brain injury (mTBI) is damage to the brain due to external forces. It is the most frequent form of brain trauma and a leading cause of disability in young adults. Hippocampal glutamatergic transmission and synaptic plasticity are impaired after mTBI, and NMDA receptors play critical in these functions. The Exocyst is a vesicle tethering complex implicated in the trafficking of glutamate receptors. We have previously shown that Exo70, a critical exocyst's subunit, redistributes in the synapse and increases its interaction with GluN2B in response to mTBI, suggesting a role in the distribution of the GluN2B subunit of NMDARs from synaptic to extrasynaptic membranes. We tested whether Exo70 could prevent NMDAR depletion from the synapse and limit mTBI pathology. To this end, we used a modified Maryland's model of mTBI in mice overexpressing Exo70 in CA1 pyramidal neurons through a lentiviral vector transduction. We showed that after mTBI, the overexpression of Exo70 prevented the cognitive impairment observed in mice infected with a control vector using the Morris' water maze paradigm. Following these findings, mice overexpressing Exo70 showed basal and NMDAR-dependent hippocampal synaptic transmission comparable to sham animals, preventing the deterioration induced by mTBI. Long-term potentiation, abundant synaptic GluN2B-containing NMDARs, and downstream signaling effectors showed that Exo70 overexpression prevented the mTBI-induced alterations. Our findings revealed a crucial role of Exo70 in NMDAR trafficking to the synapse and suggested that the Exocyst complex may be a critical component of the basal machinery that regulates NMDAR distribution in health and disease.
Description
Keywords
Traumatic brain injury, Exocyst, Exo70, NMDAR, LTP, Memory