Crosslinked oxidized-nanocellulose/chitosan hydrogels as a scaffold matrix for mesenchymal stem cell growth

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dc.contributor.authorMarino, Mayra A.
dc.contributor.authorOyarce, Karina
dc.contributor.authorTobar, Catalina
dc.contributor.authordel Rio, Rodrigo Segura
dc.contributor.authorParedes, Maria G.
dc.contributor.authorPavez, Paulina
dc.contributor.authorSarabia, Mauricio
dc.contributor.authorAmoroso, Alejandro
dc.contributor.authorConcha, Jose L.
dc.contributor.authorNorambuena-Contreras, Jose
dc.contributor.authorBarjas, Gustavo Cabrera
dc.contributor.authorCastano, Johanna
dc.date.accessioned2025-01-20T17:09:06Z
dc.date.available2025-01-20T17:09:06Z
dc.date.issued2024
dc.description.abstractMesenchymal stem cells (MSC) are recognized for their immunomodulatory effects and regenerative properties, being promising therapeutic agents for a wide range of diseases. To ensure a localized effect of MSC in the organism biobased hydrogels have been tested for their ability to act as a matrix-embedded to improve MSC targeted delivery. In this context, nanocellulose (NC) has been used for drug delivery, showing biocompatibility and durability in time, but until now NC has not been tested for MSC growth exploiting the size and aldehyde content of NC. In this study, cellulose nanocrystals (CNC), cellulose nanofibers (CNF) and microfibrillated cellulose (MFC) were studied after one-pot oxidation and further crosslinking with chitosan (mass ratio 1:5). Size and aldehyde content of oxidized NC samples were evaluated to analyze their influence on the hydrogel's properties. The crosslinked hydrogels were analyzed by FESEM, swelling ability, FTIR, compression tests, thermal stability, and stability in culture cell conditions. Oxidized-MFC hydrogel improved the mechanical stability and swelling behavior, but it lacks stability at cell conditions possibly due to its low aldehyde content (0.54 mmol/g). Conversely, oxidized CNF and oxidized CNC formed suitable crosslinked hydrogels for cell adhesion, and for growing and proliferating of MSC 3D spheroids after 120 h. However, only hydrogel with PO-CNF/chitosan shows antibacterial activity as well as MSC proliferation.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s10570-023-05591-0
dc.identifier.eissn1572-882X
dc.identifier.issn0969-0239
dc.identifier.urihttps://doi.org/10.1007/s10570-023-05591-0
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91012
dc.identifier.wosidWOS:001120841400004
dc.issue.numero1
dc.language.isoen
dc.pagina.final379
dc.pagina.inicio363
dc.revistaCellulose
dc.rightsacceso restringido
dc.subjectCrosslinked hydrogel
dc.subjectOxidized nanocellulose
dc.subjectMesenchymal stem cells
dc.titleCrosslinked oxidized-nanocellulose/chitosan hydrogels as a scaffold matrix for mesenchymal stem cell growth
dc.typeartículo
dc.volumen31
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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