Platelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells.

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dc.contributor.authorOrellana, Renan.
dc.contributor.authorKato Cardemil, Sumie Rode
dc.contributor.authorErices, Rafaela.
dc.contributor.authorBravo Castillo, María Loreto
dc.contributor.authorGonzález Hevia, Pamela Andrea.
dc.contributor.authorOliva, Bárbara.
dc.contributor.authorCubillos Alfaro, Sofía María Jesús.
dc.contributor.authorValdivia Román, Andrés Felipe
dc.contributor.authorBrañes, Jorge
dc.contributor.authorCuello F., Mauricio
dc.contributor.authorIbañez, Carolina
dc.contributor.authorBarriga Cosmelli, María Isabel
dc.contributor.authorBravo, Erasmo.
dc.contributor.authorAlonso, Catalina.
dc.contributor.authorBustamente, Eva.
dc.contributor.authorCastellon, Enrique.
dc.contributor.authorHidalgo, Patricia.
dc.contributor.authorTrigo, Cesar.
dc.contributor.authorPanes Becerra, Olga Teresa
dc.contributor.authorPereira Garcés, Jaime Ignacio
dc.contributor.authorMezzano, Diego
dc.contributor.authorOwen, Gareth Ivor
dc.date.accessioned2019-10-17T18:19:59Z
dc.date.available2019-10-17T18:19:59Z
dc.date.issued2015
dc.date.updated2019-10-14T18:35:51Z
dc.description.abstractAbstract Background An increase in circulating platelets, or thrombocytosis, is recognized as an independent risk factor of bad prognosis and metastasis in patients with ovarian cancer; however the complex role of platelets in tumor progression has not been fully elucidated. Platelet activation has been associated with an epithelial to mesenchymal transition (EMT), while Tissue Factor (TF) protein expression by cancer cells has been shown to correlate with hypercoagulable state and metastasis. The aim of this work was to determine the effect of platelet-cancer cell interaction on TF and “Metastasis Initiating Cell (MIC)” marker levels and migration in ovarian cancer cell lines and cancer cells isolated from the ascetic fluid of ovarian cancer patients. Methods With informed patient consent, ascitic fluid isolated ovarian cancer cells, cell lines and ovarian cancer spheres were co-cultivated with human platelets. TF, EMT and stem cell marker levels were determined by Western blotting, flow cytometry and RT-PCR. Cancer cell migration was determined by Boyden chambers and the scratch assay. Results The co-culture of patient-derived ovarian cancer cells with platelets causes: 1) a phenotypic change in cancer cells, 2) chemoattraction and cancer cell migration, 3) induced MIC markers (EMT/stemness), 3) increased sphere formation and 4) increased TF protein levels and activity. Conclusions We present the first evidence that platelets act as chemoattractants to cancer cells. Furthermore, platelets promote the formation of ovarian cancer spheres that express MIC markers and the metastatic protein TF. Our results suggest that platelet-cancer cell interaction plays a role in the formation of metastatic foci.
dc.fuente.origenBiomed Central
dc.identifier.citationBMC Cancer. 2015 Apr 15;15(1):290
dc.identifier.doi10.1186/s12885-015-1304-z
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26821
dc.issue.numeroNo. 290
dc.language.isoen
dc.pagina.final16
dc.pagina.inicio1
dc.revistaBMC Canceres_ES
dc.rightsacceso abierto
dc.rights.holderOrellana et al.; licensee BioMed Central.
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherCáncer de ovarioes_ES
dc.subject.otherCélulas cancerosas - Análisises_ES
dc.subject.otherMetástasises_ES
dc.titlePlatelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells.es_ES
dc.typeartículo
dc.volumenVol. 15
sipa.codpersvinculados149739
sipa.codpersvinculados62293
sipa.codpersvinculados193501
sipa.codpersvinculados76860
sipa.codpersvinculados80064
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