Artificial Symmetry-Breaking for Morphogenetic Engineering Bacterial Colonies

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dc.contributor.authorNúñez Quijada, Isaac Natán
dc.contributor.authorMatute Torres, Tamara Francisca
dc.contributor.authorDel Valle, Ilenne D.
dc.contributor.authorKan, Anton
dc.contributor.authorChoksi, Atri
dc.contributor.authorEndy, Drew
dc.contributor.authorHaseloff, Jim
dc.contributor.authorRudge, Timothy
dc.contributor.authorFederici, Fernán
dc.date.accessioned2020-05-07T21:07:15Z
dc.date.available2020-05-07T21:07:15Z
dc.date.issued2017
dc.date.updated2020-05-07T21:01:27Z
dc.description.abstractMorphogenetic engineering is an emerging field that explores the design and implementation of self-organized patterns, morphologies, and architectures in systems composed of multiple agents such as cells and swarm robots. Synthetic biology, on the other hand, aims to develop tools and formalisms that increase reproducibility, tractability, and efficiency in the engineering of biological systems. We seek to apply synthetic biology approaches to the engineering of morphologies in multicellular systems. Here, we describe the engineering of two mechanisms, symmetry-breaking and domain-specific cell regulation, as elementary functions for the prototyping of morphogenetic instructions in bacterial colonies. The former represents an artificial patterning mechanism based on plasmid segregation while the latter plays the role of artificial cell differentiation by spatial colocalization of ubiquitous and segregated components. This separation of patterning from actuation facilitates the design-build-test-improve engineering cycle. We created computational modules for CellModeller representing these basic functions and used it to guide the design process and explore the design space in silico. We applied these tools to encode spatially structured functions such as metabolic complementation, RNAPT7 gene expression, and CRISPRi/Cas9 regulation. Finally, as a proof of concept, we used CRISPRi/Cas technology to regulate cell growth by controlling methionine synthesis. These mechanisms start from single cells enabling the study of morphogenetic principles and the engineering of novel population scale structures from the bottom up.
dc.identifier.doi10.1021/acssynbio.6b00149
dc.identifier.issn2161-5063
dc.identifier.urihttp://dx.doi.org/10.1021/acssynbio.6b00149
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/28886
dc.issue.numeroNo. 2
dc.language.isoen
dc.pagina.final265
dc.pagina.inicio256
dc.relation.isformatofACS Synthetic Biology, vol. 6, no. 2 (2017), pp. 256-265.
dc.revistaACS Synthetic Biologyes_ES
dc.rightsacceso restringido
dc.subjectMorphogenetic engineeringes_ES
dc.subjectSynthetic biologyes_ES
dc.subjectMorphogenesises_ES
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleArtificial Symmetry-Breaking for Morphogenetic Engineering Bacterial Colonieses_ES
dc.typeartículo
dc.volumenVol. 6
sipa.codpersvinculados186256
sipa.codpersvinculados1031921
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