Distribution of intermediate filaments in amphibian oxyntic cells

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dc.contributor.authorDabike González, Mónica Alexandra
dc.contributor.authorKoenig, Cecilia S.
dc.contributor.authorD. Vial, Juan
dc.date.accessioned2019-12-28T02:45:15Z
dc.date.available2019-12-28T02:45:15Z
dc.date.issued1981
dc.description.abstractIntermediate filaments of toad oxyntic cells were isolated and analysed by SDS-PAGE. The major proteins of the residue were identified as actin and a 51,000 dalton polypeptide. Immunological crossreactivity between toad oxyntic cell intermediate filament components and anti-prekeratin, was shown by double immunodiffusion tests and indirect immunofluorescence. The immunofluorescent decoration of oxyntic cells and the electron microscope images are coincident in locating the intermediate filaments mainly at the cortical and perinuclear basal zones. Furthermore, the cortical zone appears especially rich in prekeratin-like material at its adluminal third. This results in a cup-like structure that encloses the cell portion occupied by the tubulovesicular system, which does not contain intermediate filaments. The translocation of membranes occurring during the secretory cycle of the oxyntic cell, has been attributed to a system of contractile proteins. The disposition of the prekeratin-like material suggests a role for intermediate filaments in the generation of movement, produced by actin and myosin interaction, by providing a fixed plane for the anchoring of actin microfilaments.
dc.format.extent12 páginas
dc.fuente.origenSpringer
dc.identifier.doi10.1007/BF00210457
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/27077
dc.identifier.urihttp://link.springer.com/article/10.1007/BF00210457
dc.issue.numeroNo. 4
dc.pagina.final737
dc.pagina.inicio725
dc.revistaCell and Tissue Researches_ES
dc.subjectOxyntic cellses_ES
dc.subjectIntermediate filamentses_ES
dc.subjectImmunofluorescencees_ES
dc.subjectPrekeratin-like filamentses_ES
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleDistribution of intermediate filaments in amphibian oxyntic cellses_ES
dc.typeartículo
dc.volumenVol. 220
sipa.codpersvinculados98811
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