Analytical detection of immunoglobulin heavy chain gene rearrangements in gastric lymphoid infiltrates by peak area analysis of the melting curve in the LightCycler System

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dc.contributor.authorRetamales, Eduardo
dc.contributor.authorRodriguez, Luis
dc.contributor.authorGuzman, Leda
dc.contributor.authorAguayo, Francisco
dc.contributor.authorPalma, Mariana
dc.contributor.authorBackhouse, Claudia
dc.contributor.authorArgandona, Jorge
dc.contributor.authorRiquelme, Erick
dc.contributor.authorCorvalan, Alejandro
dc.date.accessioned2024-01-10T13:45:44Z
dc.date.available2024-01-10T13:45:44Z
dc.date.issued2007
dc.description.abstractBecause it is difficult to differentiate gastric mucosa-associated lymphoid tissue (MALT) lymphoma from chronic gastritis in gastric lymphoid infiltrates, molecular detection of monoclonality through immunoglobulin heavy chain (IgH) gene rearrangements is commonly performed. However, heterogeneity in the performance and results obtained from IgH gene rearrangements has been reported. To improve the accuracy in the diagnosis of gastric lymphoid infiltrates, we developed an analytical approach based on one-peak area analysis of the melting curve in the LightCycler System. Using a training-testing approach, the likelihood ratio method was selected to find a discriminative function of 4.64 in the training set (10 gastric MALT lymphomas and 10 chronic gastritis cases). This discriminative function was validated in the testing set (five gastric MALT lymphomas, six abnormal lymphocytic infiltrates with subsequently demonstrated gastric MALT lymphomas, and six cases of chronic gastritis). All but one case of gastric MALT lymphoma, as well as abnormal lymphocytic infiltrates, clustered under 4.64, and all chronic gastritis cases clustered above 4.64. These results were validated by conventional electrophoreses confirming one or two sharp bands in cases of gastric MALT lymphomas and a smear of multiple bands in cases of chronic gastritis. Analytical detection of IgH gene rearrangement in gastric lymphoid infiltrates by one-peak area analysis correctly distinguishes gastric MALT lymphomas from chronic gastritis, even in cases with diagnosis of abnormal lymphocytic infiltrates.
dc.fechaingreso.objetodigital2024-05-22
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.2353/jmoldx.2007.050129
dc.identifier.eissn1943-7811
dc.identifier.issn1525-1578
dc.identifier.pubmedidMEDLINE:17591935
dc.identifier.urihttps://doi.org/10.2353/jmoldx.2007.050129
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79073
dc.identifier.wosidWOS:000247691200011
dc.information.autorucMedicina;Corvalan A;S/I;63885
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final357
dc.pagina.inicio351
dc.publisherELSEVIER SCIENCE INC
dc.revistaJOURNAL OF MOLECULAR DIAGNOSTICS
dc.rightsacceso restringido
dc.subjectENDOSCOPIC BIOPSY SPECIMENS
dc.subjectRAPID DIAGNOSIS
dc.subjectHYBRIDIZATION
dc.subjectCLONALITY
dc.subjectPCR
dc.subjectCLASSIFICATION
dc.subjectMONOCLONALITY
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAnalytical detection of immunoglobulin heavy chain gene rearrangements in gastric lymphoid infiltrates by peak area analysis of the melting curve in the LightCycler System
dc.typeartículo
dc.volumen9
sipa.codpersvinculados63885
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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