Spatio-temporal expression of MMP-2, MMP-9 and tissue kallikrein in uteroplacental units of the pregnant guinea-pig (Cavia porcellus)

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dc.contributor.authorCorthorn H., Jenny
dc.contributor.authorRey Keim, Sergio
dc.contributor.authorChacón Figueroa, Cynthia Cecilia.
dc.contributor.authorValdés Stromilli, Gloria
dc.date.accessioned2019-10-17T18:19:55Z
dc.date.available2019-10-17T18:19:55Z
dc.date.issued2007
dc.date.updated2019-10-14T18:45:27Z
dc.description.abstractAbstract Background In humans trophoblast invasion and vascular remodeling are critical to determine the fate of pregnancy. Since guinea-pigs share with women an extensive migration of the trophoblasts through the decidua and uterine arteries, and a haemomonochorial placenta, this species was used to evaluate the spatio-temporal expression of three enzymes that have been associated to trophoblast invasion, MMP-2, MMP-9 and tissue kallikrein (K1). Methods Uteroplacental units were collected from early to term pregnancy. MMP-2, MMP-9 and K1 were analysed by immunohistochemistry and Western blot. The activities of MMP-2 and MMP-9 were assessed by gelatin zymography. Results Immunoreactive MMP-2, MMP-9 and K1 were detected in the subplacenta, interlobar and labyrinthine placenta, syncytial sprouts and syncytial streamers throughout pregnancy. In late pregnancy, perivascular or intramural trophoblasts expressed the three enzymes. The intensity of the signal in syncytial streamers was increased in mid and late pregnancy for MMP-2, decreased in late pregnancy for MMP-9, and remained stable for K1. Western blots of placental homogenates at days 20, 40 and 60 of pregnancy identified bands with the molecular weights of MMP-2, MMP-9 and K1. MMP-2 expression remained constant throughout gestation. In contrast, MMP-9 and K1 attained their highest expression during midgestation. Placental homogenates of 20, 40 and 60 days yielded bands of gelatinase activity that were compatible with MMP-2 and MMP-9 activities. ProMMP-2 and MMP-9 activities did not vary along pregnancy, while MMP-2 and MMP-9 increased at 40 and 40–60 days respectively. Conclusion The spatio-temporal expression of MMPs and K1 supports a relevant role of these proteins in trophoblast invasion, vascular remodeling and placental angiogenesis, and suggests a functional association between K1 and MMP-9 activation.
dc.fuente.origenBiomed Central
dc.identifier.citationReproductive Biology and Endocrinology. 2007 Jul 02;5(1):27
dc.identifier.doi10.1186/1477-7827-5-27
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26820
dc.issue.numeroNo.27
dc.language.isoen
dc.pagina.final13
dc.pagina.inicio1
dc.revistaReproductive Biology and Endocrinologyes_ES
dc.rightsacceso abierto
dc.rights.holderCorthorn et al; licensee BioMed Central Ltd.
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherCélulas - Análisis - Investigacioneses_ES
dc.subject.otherBiología - Investigacioneses_ES
dc.subject.otherEmbarazo - Diagnósticoes_ES
dc.titleSpatio-temporal expression of MMP-2, MMP-9 and tissue kallikrein in uteroplacental units of the pregnant guinea-pig (Cavia porcellus)es_ES
dc.typeartículo
dc.volumenVol.5
sipa.codpersvinculados102180
sipa.codpersvinculados1083
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