Enhanced DNA extraction and PCR amplification of SSU ribosomal genes from crustose coralline algae

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dc.contributor.authorVidal, R
dc.contributor.authorMeneses, I
dc.contributor.authorSmith, M
dc.date.accessioned2025-01-21T01:30:25Z
dc.date.available2025-01-21T01:30:25Z
dc.date.issued2002
dc.description.abstractAs crustose Corallinales on the coasts of Chile are usually flat, thin, strongly adherent to the rocks and with a high concentration of polysaccharides, there is a need to improve DNA extraction for molecular studies. The paper describes a protocol to achieve this, which includes steps for disruption of cell walls and precipitation of polysaccharides and proteins; this leads to a PCR-quality product. The DNA obtained permitted amplification of SSU and rbcL genes from small amounts of material (< 1 g) of several genera and species of Corallinales. Comparison of the sequences of small SSU fragments (approximately 500 bp), combined with morphological characters, together provide sufficient resolution to distinguish organisms at the genus and species levels.
dc.fuente.origenWOS
dc.identifier.eissn1573-5176
dc.identifier.issn0921-8971
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96744
dc.identifier.wosidWOS:000177722000010
dc.issue.numero3
dc.language.isoen
dc.pagina.final227
dc.pagina.inicio223
dc.revistaJournal of applied phycology
dc.rightsacceso restringido
dc.subjectcrustose coralline algae
dc.subjectDNA extraction
dc.subjectpolysaccharide inhibition
dc.subjectsmall subunit RNA (SSU)
dc.subject.ods14 Life Below Water
dc.subject.ods13 Climate Action
dc.subject.ods15 Life on Land
dc.subject.odspa14 Vida submarina
dc.subject.odspa13 Acción por el clima
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleEnhanced DNA extraction and PCR amplification of SSU ribosomal genes from crustose coralline algae
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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