Noninvasive Magnetic Resonance Imaging Evaluation of Endothelial Permeability in Murine Atherosclerosis Using an Albumin-Binding Contrast Agent

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dc.contributor.authorPhinikaridou, Alkystis
dc.contributor.authorAndia, Marcelo E.
dc.contributor.authorProtti, Andrea
dc.contributor.authorIndermuchle, Andreas
dc.contributor.authorShah, Ajay
dc.contributor.authorSmith, Alberto
dc.contributor.authorWarley, Alice
dc.contributor.authorBotnar, Rene M.
dc.date.accessioned2024-01-10T12:07:00Z
dc.date.available2024-01-10T12:07:00Z
dc.date.issued2012
dc.description.abstractBackgound-Endothelial dysfunction promotes atherosclerosis and precedes acute cardiovascular events. We investigated wether in vivo magnetic resonance imaging with the use of an albumin-binding contrast agent, gadofosveset, could detect endothelial damage associated with atherosclerosis in apolipoprotein E-deficient (ApoE(-/-)) mice. Furthermore, we tested whether magnetic resonance imaging could noninvasively assess endothelial function by measuring the endothelial-dependent vasolidation in response to acetycholine.
dc.description.abstractMethods and Results-ApoE(-/-) mice were imaged at 4, 8, and 12 weeks after commencement of a high-fat diet. Statin-treated ApoE(-/-) mice were scanned after 12 weeks of a high-fat diet. Wild-type mice were imaged before and 48 hours after injection of Russell's viper venom, an endothelial toxin. Delayed enhancement magnetic resonance imaging and T1 mapping of the brachiocephalic artery, 30 minutes after injection of gadofosveset, showed increased vessel wall enhancement and relaxation rate (R-1) with progression of atherosclerosis in ApoE(-/-) (R-1 [s(-1)]: R-4 (weeks) 2.42 +/- 0.35, R-8 (weeks) 3.45 +/- 0.54, R-12 (weeks) 3.83 +/- 0.52) and Russell's viper venom-injected wild-type mice (R-1=4.57 +/- 0.86). Conversely, wild-type (R-1=2.15 +/- 0.34) and statin-treated ApoE(-/-) (R-1=3.0 +/- 0.65) mice showed less enhancement. Uptake of gadofosveset correlated with Evans blue staining, morphological changes of endothelial cells, and widening of the cell-cell junctions, suggesting that uptake occurs in regions of increased vascular permeability. Endothelial-dependent vasomotor responses showed vasoconstriction of the arteries of the ApoE(-/-) (-22.22 +/- 7.95%) and Russell's viper venom-injected (-10.37 +/- 17.60%) mice compared with wild-type mice (32.45 +/- 12.35%), Statin treatment improved endothelium morphology and function (-8.12 +/- 8.22%).
dc.description.abstractConclusions-We demonstrate the noninvasive assessment of endothelial permeability and function with the use of an albumin-binding magnetic resonance contrast agent. Blood albumin leakage could be a surrogate marker for the in vivo evaluaion of intervention that aim to restore the endothelium. (Circulation. 2012;126:707-719.)
dc.description.funderBritish Heart Foundation
dc.description.funderBritish Heart Foundation
dc.fechaingreso.objetodigital2024-05-20
dc.format.extent18 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1161/CIRCULATIONAHA.112.092098
dc.identifier.eissn1524-4539
dc.identifier.issn0009-7322
dc.identifier.pubmedidMEDLINE:22753191
dc.identifier.urihttps://doi.org/10.1161/CIRCULATIONAHA.112.092098
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76233
dc.identifier.wosidWOS:000307472600017
dc.information.autorucMedicina;Andia M;S/I;90691
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.publisherLIPPINCOTT WILLIAMS & WILKINS
dc.revistaCIRCULATION
dc.rightsacceso restringido
dc.subjectatherosclerosis
dc.subjectendothelial dysfunction
dc.subjectgadofosveset
dc.subjectmagnetic resonance imaging
dc.subjectpermeability
dc.subjectNORMAL CORONARY-ARTERIES
dc.subjectIN-VIVO
dc.subjectCHOLESTEROL DIET
dc.subjectDEFICIENT MICE
dc.subjectVESSEL WALL
dc.subjectDYSFUNCTION
dc.subjectVISUALIZATION
dc.subjectLESIONS
dc.subjectVASODILATION
dc.subjectANGIOGENESIS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNoninvasive Magnetic Resonance Imaging Evaluation of Endothelial Permeability in Murine Atherosclerosis Using an Albumin-Binding Contrast Agent
dc.typeartículo
dc.volumen126
sipa.codpersvinculados90691
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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