Novel polymerase chain reaction primers for the specific detection of bacterial copper P-type ATPases gene sequences in environmental isolates and metagenomic DNA

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dc.contributor.authorDe la Iglesia, R.
dc.contributor.authorValenzuela Heredia, D.
dc.contributor.authorPavissich, J. P.
dc.contributor.authorFreyhoffer, S.
dc.contributor.authorAndrade, S.
dc.contributor.authorCorrea, J. A.
dc.contributor.authorGonzalez, B.
dc.date.accessioned2024-01-10T13:15:38Z
dc.date.available2024-01-10T13:15:38Z
dc.date.issued2010
dc.description.abstractAims:
dc.description.abstractIn the last decades, the worldwide increase in copper wastes release by industrial activities like mining has driven environmental metal contents to toxic levels. For this reason, the study of the biological copper-resistance mechanisms in natural environments is important. Therefore, an appropriate molecular tool for the detection and tracking of copper-resistance genes was developed.
dc.description.abstractMethods and Results:
dc.description.abstractIn this work, we designed a PCR primer pair to specifically detect copper P-type ATPases gene sequences. These PCR primers were tested in bacterial isolates and metagenomic DNA from intertidal marine environments impacted by copper pollution. As well, T-RFLP fingerprinting of these gene sequences was used to compare the genetic composition of such genes in microbial communities, in normal and copper-polluted coastal environments. New copper P-type ATPases gene sequences were found, and a high degree of change in the genetic composition because of copper exposure was also determined.
dc.description.abstractConclusions:
dc.description.abstractThis PCR based method is useful to track bacterial copper-resistance gene sequences in the environment.
dc.description.abstractSignificance and Impact of the Study:
dc.description.abstractThis study is the first to report the design and use of a PCR primer pair as a molecular marker to track bacterial copper-resistance determinants, providing an excellent tool for long-term analysis of environmental communities exposed to metal pollution.
dc.description.funderCONICYT
dc.description.funderMillennium Scientific Initiative
dc.description.funderMarine Genomics-CONICYT, FUNDACION ANDES
dc.description.funderPCBT
dc.fechaingreso.objetodigital2024-05-09
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1111/j.1472-765X.2010.02832.x
dc.identifier.eissn1472-765X
dc.identifier.issn0266-8254
dc.identifier.pubmedidMEDLINE:20337927
dc.identifier.urihttps://doi.org/10.1111/j.1472-765X.2010.02832.x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78518
dc.identifier.wosidWOS:000277417000002
dc.information.autorucCiencias Biológicas;Andrade S;S/I;163520
dc.information.autorucCiencias Biológicas;Correa JA;S/I;54626
dc.information.autorucCiencias Biológicas;De la Iglesia R;S/I;13915
dc.information.autorucCiencias Biológicas;Pavissich JP;S/I;120114
dc.information.autorucCiencias Biológicas;Valenzuela-Heredia D;S/I;132604
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final562
dc.pagina.inicio552
dc.publisherWILEY
dc.revistaLETTERS IN APPLIED MICROBIOLOGY
dc.rightsacceso restringido
dc.subjectATPases
dc.subjectbacterial communities
dc.subjectcopper resistance
dc.subjectmetagenomic DNA
dc.subjectPCR primer pair
dc.subjectHEAVY-METAL RESISTANCE
dc.subjectCOMMUNITY STRUCTURE
dc.subjectESCHERICHIA-COLI
dc.subjectNORTHERN CHILE
dc.subjectMINE TAILINGS
dc.subjectHOMEOSTASIS
dc.subjectDIVERSITY
dc.subjectCOPA
dc.subjectTRANSPORT
dc.subjectTOXICITY
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNovel polymerase chain reaction primers for the specific detection of bacterial copper P-type ATPases gene sequences in environmental isolates and metagenomic DNA
dc.typeartículo
dc.volumen50
sipa.codpersvinculados163520
sipa.codpersvinculados54626
sipa.codpersvinculados13915
sipa.codpersvinculados120114
sipa.codpersvinculados132604
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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