GPR43 stimulation on TCRαβ+ intraepithelial colonic lymphocytes inhibits the recruitment of encephalitogenic T-cells into the central nervous system and attenuates the development of autoimmunity

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dc.article.number135
dc.catalogadorcrc
dc.contributor.authorPrado, Carolina
dc.contributor.authorEspinoza, Alexandra
dc.contributor.authorMartínez-Hernández, J. E.
dc.contributor.authorPetrosino, Joseph
dc.contributor.authorRiquelme Sánchez, Erick Marcelo
dc.contributor.authorMartin, Alberto J. M.
dc.contributor.authorPacheco, Rodrigo
dc.date.accessioned2023-06-05T15:03:19Z
dc.date.available2023-06-05T15:03:19Z
dc.date.issued2023
dc.date.updated2023-06-04T00:02:44Z
dc.description.abstractAbstract Introduction Gut microbiota plays a critical role in the regulation of immune homeostasis. Accordingly, several autoimmune disorders have been associated with dysbiosis in the gut microbiota. Notably, the dysbiosis associated with central nervous system (CNS) autoimmunity involves a substantial reduction of bacteria belonging to Clostridia clusters IV and XIVa, which constitute major producers of short-chain fatty acids (SCFAs). Here we addressed the role of the surface receptor-mediated effects of SCFAs on mucosal T-cells in the development of CNS autoimmunity. Methods To induce CNS autoimmunity, we used the mouse model of experimental autoimmune encephalomyelitis (EAE) induced by immunization with the myelin oligodendrocyte glycoprotein (MOG)-derived peptide (MOG35-55 peptide). To address the effects of GPR43 stimulation on colonic TCRαβ+ T-cells upon CNS autoimmunity, mucosal lymphocytes were isolated and stimulated with a selective GPR43 agonist ex vivo and then transferred into congenic mice undergoing EAE. Several subsets of lymphocytes infiltrating the CNS or those present in the gut epithelium and gut lamina propria were analysed by flow cytometry. In vitro migration assays were conducted with mucosal T-cells using transwells. Results Our results show a sharp and selective reduction of intestinal propionate at the peak of EAE development, accompanied by increased IFN-γ and decreased IL-22 in the colonic mucosa. Further analyses indicated that GPR43 was the primary SCFAs receptor expressed on T-cells, which was downregulated on colonic TCRαβ+ T-cells upon CNS autoimmunity. The pharmacologic stimulation of GPR43 increased the anti-inflammatory function and reduced the pro-inflammatory features in several TCRαβ+ T-cell subsets in the colonic mucosa upon EAE development. Furthermore, GPR43 stimulation induced the arrest of CNS-autoreactive T-cells in the colonic lamina propria, thus avoiding their infiltration into the CNS and dampening the disease development. Mechanistic analyses revealed that GPR43-stimulation on mucosal TCRαβ+ T-cells inhibits their CXCR3-mediated migration towards CXCL11, which is released from the CNS upon neuroinflammation. Conclusions These findings provide a novel mechanism involved in the gut-brain axis by which bacterial-derived products secreted in the gut mucosa might control the CNS tropism of autoreactive T-cells. Moreover, this study shows GPR43 expressed on T-cells as a promising therapeutic target for CNS autoimmunity.
dc.fechaingreso.objetodigital2023-06-04
dc.fuente.origenAutoarchivo
dc.identifier.citationJournal of Neuroinflammation. 2023 Jun 01;20(1):135
dc.identifier.issn1742-2094
dc.identifier.urihttps://doi.org/10.1186/s12974-023-02815-9
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/70518
dc.information.autorucEscuela de Medicina; Riquelme Sanchez Erick Marcelo; s/i;1001194
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final19
dc.pagina.inicio1
dc.revistaJournal of Neuroinflammation
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.rights.licenseCC0 1.0 Universal (CC0 1.0)
dc.rights.urihttps://creativecommons.org/publicdomain/zero/1.0/deed.es
dc.subjectShort-chain fatty acids
dc.subjectGPR43
dc.subjectMucosal immunity
dc.subjectGut-brain axis
dc.subjectNeuroinflammation
dc.subjectExperimental autoimmune encephalomyelitis
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleGPR43 stimulation on TCRαβ+ intraepithelial colonic lymphocytes inhibits the recruitment of encephalitogenic T-cells into the central nervous system and attenuates the development of autoimmunity
dc.typeartículo
dc.volumen20
sipa.codpersvinculados1001194
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