Evaluation of ELISA-based multiplex peptides for the detection of human serum antibodies induced by zika virus infection across various countries

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dc.article.number1319
dc.catalogadorpva
dc.contributor.authorViedma, M.D.P.M.
dc.contributor.authorPanossian, S.
dc.contributor.authorGifford, K.
dc.contributor.authorGarcía, K.
dc.contributor.authorFigueroa, I.
dc.contributor.authorParham, L.
dc.contributor.authorde Moraes, L.
dc.contributor.authorGomes, L.N.
dc.contributor.authorGarcía-Salum, T.
dc.contributor.authorPerret, C.
dc.contributor.authorWeiskopf, D.
dc.contributor.authorTan, G.S.
dc.contributor.authorSilva, A.A.
dc.contributor.authorBoaventura, V.
dc.contributor.authorRuiz-Palacios, G.M.
dc.contributor.authorSette, A.
dc.contributor.authorDe Silva, A.D.
dc.contributor.authorMedina, R.A.
dc.contributor.authorLorenzana, I.
dc.contributor.authorAkrami, K.M.
dc.contributor.authorKhouri, R.
dc.contributor.authorOlson, D.
dc.contributor.authorPickett, B.E.
dc.date.accessioned2024-03-04T15:19:38Z
dc.date.available2024-03-04T15:19:38Z
dc.date.issued2021
dc.description.abstractZika virus (ZIKV) is a mosquito-borne Flavivirus with a positive-sense RNA genome, which are generally transmitted through the bite of an infected Aedes mosquito. ZIKV infections could be associated with neurological sequelae that, and otherwise produces similar clinical symptoms as other co-circulating pathogens. Past infection with one member of the Flavivirus genus often induces cross-reactive antibodies against other flaviruses. These attributes complicate the ability to differentially diagnose ZIKV infection from other endemic mosquito-borne viruses, making it both a public health issue as well as a diagnostic challenge. We report the results from serological analyses using arbovirus-specific peptides on 339 samples that were previously collected from 6 countries. Overall, we found that our multiplexed peptide-based ELISA was highly efficient for identifying ZIKV antibodies as early as 2 weeks post infection, and that it correlates with microneutralization, plaque reduction neutralization tests (PRNTs) and commercial tests for ZIKV in previously characterized samples. We observed that seropositivity varied by patient cohort, reflecting the sampling period in relation to the 2015–2016 ZIKV outbreak. This work evaluates the accuracy, specificity, and sensitivity of our peptide-based ELISA method for detecting ZIKV antibodies from geographically diverse regions. These findings can contribute to ongoing serological methods development and can be adapted for use in future studies.
dc.fechaingreso.objetodigital2024-03-25
dc.format.extent15 páginas
dc.fuente.origenORCID
dc.identifier.doi10.3390/v13071319
dc.identifier.eissn1999-4915
dc.identifier.urihttps://doi.org/10.3390/v13071319
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/82443
dc.information.autorucEscuela de Medicina; Perret Perez, Cecilia; 0000-0002-1535-1204; 80387
dc.issue.numero7
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final15
dc.pagina.inicio1
dc.revistaViruses
dc.rightsacceso abierto
dc.rights.licenseCC BY 4.0 DEED Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectZika virus
dc.subjectAntibody diagnostic
dc.subjectSeropositivity
dc.subjectELISA
dc.subjectVirology
dc.subjectBioinformatics
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleEvaluation of ELISA-based multiplex peptides for the detection of human serum antibodies induced by zika virus infection across various countries
dc.typeartículo
dc.volumen13
sipa.codpersvinculados80387
sipa.trazabilidadORCID;2024-01-15
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