Metabolic engineering of Corynebacterium glutamicum for trehalose overproduction: Role of the TreYZ trehalose biosynthetic pathway

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dc.contributor.authorCarpinelli, J
dc.contributor.authorKramer, R
dc.contributor.authorAgosin, E
dc.date.accessioned2024-01-10T13:14:49Z
dc.date.available2024-01-10T13:14:49Z
dc.date.issued2006
dc.description.abstractTrehalose has many potential applications in biotechnology and the food industry due to its protective effect against environmental stress. Our work explores microbiological production methods based on the capacity of Corynebacterium glutainicum to excrete trehalose. We address here raising trehalose productivity through homologous overexpression of maltooligosyltrehalose synthase and the maltooligosyltrehallose trehalohydrolase genes. In addition, heterologous expression of the UDP-glucose pyrophosphorylase gene from Escherichia coli improved the supply of glycogen. Gene expression effects were tested on enzymatic activities and intracellular glycogen content, as well as on accumulated and excreted trehalose. Overexpression of the treY gene and the treY/treZ synthetic operon significantly increased maltooligosyltrehalose synthase activity, the rate-limiting step, and improved the specific productivity and the final titer of trehalose. Furthermore, a strong decrease was noted in glycogen accumulation. Expression of galU/treY and galU/treYZ synthetic operons showed a partial recovery in the intracellular glycogen levels and a significant improvement in both intra- and extracellular trehalose content.
dc.fechaingreso.objetodigital2024-05-14
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1128/AEM.72.3.1949-1955.2006
dc.identifier.eissn1098-5336
dc.identifier.issn0099-2240
dc.identifier.pubmedidMEDLINE:16517642
dc.identifier.urihttps://doi.org/10.1128/AEM.72.3.1949-1955.2006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78443
dc.identifier.wosidWOS:000236069200028
dc.information.autorucIngeniería;Agosin E;S/I;99630
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1955
dc.pagina.inicio1949
dc.publisherAMER SOC MICROBIOLOGY
dc.revistaAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
dc.rightsacceso restringido
dc.subjectUDP-GLUCOSE PYROPHOSPHORYLASE
dc.subjectESCHERICHIA-COLI
dc.subjectHETEROLOGOUS EXPRESSION
dc.subjectGROWTH
dc.subjectTRANSFORMATION
dc.subjectSYNTHASE
dc.subjectGLYCOGEN
dc.subjectFLUX
dc.subjectDNA
dc.subject.ods12 Responsible consuption and production
dc.subject.odspa12 Producción y consumo responsable
dc.titleMetabolic engineering of Corynebacterium glutamicum for trehalose overproduction: Role of the TreYZ trehalose biosynthetic pathway
dc.typeartículo
dc.volumen72
sipa.codpersvinculados99630
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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