AFM Study of Elastic Module of Physical-Vapor-Deposited Phospholipid Membranes

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dc.catalogadorjlo
dc.contributor.authorVolkmann, Ulrich Georg
dc.contributor.authorCatalán López, Rodrigo Esteban
dc.contributor.authorRetamal Ponce, María José
dc.contributor.authorCisternas Fruns, Marcelo Andrés
dc.contributor.authorMoraga Vent, Nicolás Andrés
dc.contributor.authorDíaz Díaz, Diego Ignacio
dc.contributor.authorCorrales, Tomás P.
dc.contributor.authorPérez-Acle, Tomás
dc.contributor.authorSoto Arriaza, Marco Antonio
dc.contributor.authorHuber, Patrick
dc.date.accessioned2024-04-23T16:02:45Z
dc.date.available2024-04-23T16:02:45Z
dc.date.issued2018
dc.description.abstractThe physical study of artificial phospholipid membranes on solid substrates has become a relevant way to gain insights into the physical behavior of cell membranes. The study of mechanical properties of artificial membranes (lab-made) has become possible with the use of atomic force microscopy. Here, we analyze the Young's modulus and adhesion force of several phospholipidic membranes as a function of temperature using Scanning Force Spectroscopy (SFS). Phospholipids were deposited onto double-polished silicon substrates by physical vapor deposition (PVD), as we have recently reported [1, 2]. We have used Raman spectroscopy to show that the chemical structure of our deposited phospholipid remains unaltered after PVD. After PVD we performed AFM and SFS measurements on the following phospholipids: DPPC, DMPC and DSPC. Measurements using AFM in liquid confirm the self-assembly of the phospholipid bilayer. By using SFS of the deposited membrane in liquid, we observe 2 phase transitions, both in Younǵs Modulus, as well as adhesion channels. The measured phase transitions are consistent with the ripple-gel transition and the gel-liquid crystalline phases. Furthermore, we have studied the frequency dependence of these phase transitions by changing the tip velocity in SFS experiments. Supported by FONDECYT grant # 1141105 (UGV), FONDECYT INICIACION grant # 11160664 (TPC), CONICYT Fellowships (RC and MC), Postdoctoral Fellowship FONDECYT 3160803 (MJR) and CONICYT-PIA ACT 1409. [1] María J. Retamal, Marcelo A. Cisternas, Sebastian E. Gutierrez-Maldonado, Tomas Perez-Acle, Birger Seifert, Mark Busch, Patrick Huber and Ulrich G. Volkmann, J. Chem. Phys. 141, 104201 (2014). [2] María Jose Retamal, Tomas P. Corrales, Marcelo A. Cisternas, Nicolas H. Moraga, Diego I. Diaz, Rodrigo E. Catalan, Birger Seifert, Patrick Huber, and Ulrich G. Volkmann. Biomacromolecules 17 (3), 1142 (2016).
dc.description.funderFONDECYT
dc.fuente.origenHistorial Académico
dc.identifier.citationVolkmann, Ulrich Georg, Catalán, Rodrigo, Retamal, María José, Cisternas, Marcelo A, Moraga, Nicolas, Díaz, Diego, Corrales, Tomás P, Párez-Acle, Tomás, Soto-Arriaza, Marco, Huber, Patrick. AFM Study of Elastic Module of Physical-Vapor-Deposited Phospholipid Membranes. Biophysical Journal. 2018;114:105A-105A.
dc.identifier.doi10.1016/J.BPJ.2017.11.614
dc.identifier.issn0006-3495
dc.identifier.urihttps://doi.org/10.1016/j.bpj.2017.11.614
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/85286
dc.identifier.wosidWOS:000429315800538
dc.information.autorucInstituto de Física; Volkmann, Ulrich Georg; 0000-0003-4972-5558; 100470
dc.information.autorucInstituto de Física; Catalán López, Rodrigo Esteban; 0000-0002-4126-3240; 186652
dc.information.autorucInstituto de Física; Retamal Ponce, María José; S/I; 140896
dc.information.autorucInstituto de Física; Cisternas Fruns, Marcelo Andrés; 0000-0002-8612-8560; 148226
dc.information.autorucInstituto de Física; Moraga Vent, Nicolás Andrés; 0000-0002-9423-1170; 1070555
dc.information.autorucInstituto de Física; Díaz Díaz, Diego Ignacio; S/I; 185252
dc.information.autorucEscuela de Química; Soto Arriaza, Marco Antonio; 0000-0003-4502-6960; 160201
dc.issue.numero3
dc.language.isoen
dc.nota.accesosin adjunto
dc.revistaBiophysical Journal
dc.rightsregistro bibliográfico
dc.subject.ddc510
dc.subject.deweyMatemática física y químicaes_ES
dc.titleAFM Study of Elastic Module of Physical-Vapor-Deposited Phospholipid Membranes
dc.typeartículo
dc.volumen114
sipa.codpersvinculados131149
sipa.codpersvinculados1010271
sipa.codpersvinculados185222
sipa.codpersvinculados100470
sipa.codpersvinculados186652
sipa.codpersvinculados140896
sipa.codpersvinculados148226
sipa.codpersvinculados1070555
sipa.codpersvinculados185252
sipa.codpersvinculados160201
sipa.trazabilidadHistorial Académico;09-07-2021
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