Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs <i>34a</i> and <i>-let-7c</i> in Canine Follicles during the Estrous Cycle

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dc.contributor.authorDe los Reyes, Monica
dc.contributor.authorDettleff, Phillip
dc.contributor.authorPalomino, Jaime
dc.contributor.authorPeralta, Oscar A.
dc.contributor.authorVergara, Ana
dc.date.accessioned2025-01-20T17:09:34Z
dc.date.available2025-01-20T17:09:34Z
dc.date.issued2024
dc.description.abstractThe genes encoding for estrogen receptor (ESR2) and follicle-stimulating hormone receptor (FSHR) play crucial roles in ovarian follicular development. This study aimed to determine the expression levels of miRNAs predicted against FSHR and ESR2 mRNAs in follicular cells related to their target genes during the estrous cycle in canines. Antral follicles were dissected from 72 ovaries following ovariohysterectomies. MiRNAs regulating FSHR and ESR2 genes were selected from miRNA databases, and mature miRNA and mRNA expression profiling was performed using real-time polymerase chain reaction (PCR). The best miRNA for each target gene was selected considering the quantitative PCR (qPCR) performance and target prediction probability, selecting only miRNAs with a binding p-value of 1.0, and choosing cfa-miR-34a and cfa-let-7c for FSHR and ESR2, respectively. The expression levels comparing the different phases of the estrous cycle were evaluated using ANOVA. Pearson correlations between the expression pattern of each miRNA and their target genes were performed. Each miRNA and its target genes were expressed in the granulosa cells in all estrous phases. FSHR remained low in anestrus and proestrus, increased (p < 0.05) to the highest level in estrus, and decreased (p < 0.05) in diestrus. ESR2 showed the same trend as FSHR, with the highest (p < 0.05) expression in estrus and the lowest (p < 0.05) in anestrus and proestrus. A tendency for an inverse relationship was observed between the expression of miR-34a and FSHR only in the anestrus phase, while an inverse correlation (r = -0.8) was found between miRNA-7c and ESR2 (p < 0.01). The expression profile of miR-34a and miR-let-7c and their predicted target genes of dog ovarian follicles throughout the estrous cycle observed in this study suggest a role in the transcriptional regulation of FSHR and ESR2, which is the first evidence of the involvement of these miRNAs in the canine follicular function.
dc.fuente.origenWOS
dc.identifier.doi10.3390/ani14020214
dc.identifier.issn2076-2615
dc.identifier.urihttps://doi.org/10.3390/ani14020214
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91054
dc.identifier.wosidWOS:001151889900001
dc.issue.numero2
dc.language.isoen
dc.revistaAnimals
dc.rightsacceso restringido
dc.subjectdog
dc.subjectgene expression
dc.subjectovarian cycle
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleDynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs <i>34a</i> and <i>-let-7c</i> in Canine Follicles during the Estrous Cycle
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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