Human platelets synthesize and express functional tissue factor

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dc.contributor.authorPanes, Olga
dc.contributor.authorMatus, Valeria
dc.contributor.authorSaez, Claudia G.
dc.contributor.authorQuiroga, Teresa
dc.contributor.authorPereira, Jaime
dc.contributor.authorMezzano, Diego
dc.date.accessioned2024-01-10T13:49:10Z
dc.date.available2024-01-10T13:49:10Z
dc.date.issued2007
dc.description.abstractThe source and significance of bloodborne tissue factor (TF) are controversial. TF mRNA, protein, and TF-dependent procoagulant activity (PCA) have been detected in human platelets, but direct evidence of TF synthesis is missing. Nonstimulated monocyte-free platelets from most patients expressed TF mRNA, which was enhanced or induced in all of them after platelet activation. Immunoprecipitation assays revealed TF protein (mainly of a molecular weight [Mr] of approximately 47 kDa, with other bands of approximately 35 and approximately 60 kDa) in nonstimulated platelet membranes, which also increased after activation. This enhancement was concomitant with TF translocation to the plasma membrane, as demonstrated by immunofluorescence-confocal microscopy and biotinylation of membrane proteins. Platelet PCA, assessed by factor Xa (FXa) generation, was induced after activation and was inhibited by 48% and 76% with anti-TF and anti-FVIIa, respectively, but not by intrinsic pathway inhibitors. Platelets incorporated [35S]-methionine into TF proteins with Mr of approximately 47 kDa, approximately 35 kDa, and approximately 60 kDa, more intensely after activation. Puromycin but not actinomycin D or DRB (5,6-dichloro-1 -beta-D-ribofuranosylbenzimidazole)inhibited TIF neosynthesis. Thus, human platelets not only assemble the clotting reactions on their membrane, but also supply their own TIF for thrombin generation in a timely and spatially circumscribed process. These observations simplify, unify, and provide a more coherent formulation of the current cellbased model of hemostasis.
dc.fechaingreso.objetodigital2024-05-15
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1182/blood-2006-06-030619
dc.identifier.eissn1528-0020
dc.identifier.issn0006-4971
dc.identifier.pubmedidMEDLINE:17347408
dc.identifier.urihttps://doi.org/10.1182/blood-2006-06-030619
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79428
dc.identifier.wosidWOS:000247360200037
dc.information.autorucMedicina;Matus V;S/I;1744
dc.information.autorucMedicina;Mezzano D;S/I;99455
dc.information.autorucMedicina;Panes O;S/I;90271
dc.information.autorucMedicina;Pereira J;S/I;99371
dc.information.autorucMedicina;Saez CG;S/I;102375
dc.issue.numero12
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final5250
dc.pagina.inicio5242
dc.publisherAMER SOC HEMATOLOGY
dc.revistaBLOOD
dc.rightsacceso restringido
dc.subjectHUMAN ENDOTHELIAL-CELLS
dc.subjectFACTOR MESSENGER-RNA
dc.subjectACTIVATED PLATELETS
dc.subjectPLASMINOGEN-ACTIVATOR
dc.subjectFACTOR CONTRIBUTES
dc.subjectLIPID RAFTS
dc.subjectP-SELECTIN
dc.subjectFACTOR-VII
dc.subjectCOAGULATION
dc.subjectTHROMBIN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHuman platelets synthesize and express functional tissue factor
dc.typeartículo
dc.volumen109
sipa.codpersvinculados1744
sipa.codpersvinculados99455
sipa.codpersvinculados90271
sipa.codpersvinculados99371
sipa.codpersvinculados102375
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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