The 5'-untranslated region of the mouse mammary tumor virus mRNA exhibits cap-independent translation initiation

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dc.contributor.authorVallejos, Maricarmen
dc.contributor.authorRamdohr, Pablo
dc.contributor.authorValiente Echeverria, Fernando
dc.contributor.authorTapia, Karla
dc.contributor.authorRodriguez, Felipe E.
dc.contributor.authorLowy, Fernando
dc.contributor.authorPablo Huidobro Toro, J.
dc.contributor.authorDangerfield, John A.
dc.contributor.authorLopez Lastra, Marcelo
dc.date.accessioned2024-01-10T14:21:53Z
dc.date.available2024-01-10T14:21:53Z
dc.date.issued2010
dc.description.abstractIn this study, we demonstrate the identification of an internal ribosome entry site (IRES) within the 5'-untranslated region (5'-UTR) of the mouse mammary tumor virus (MMTV). The 5'-UTR of the full-length mRNA derived from the infectious, complete MMTV genome was cloned into a dual luciferase reporter construct containing an upstream Renilla luciferase gene (RLuc) and a downstream firefly luciferase gene (FLuc). In rabbit reticulocyte lysate, the MMTV 5'-UTR was capable of driving translation of the second cistron. In vitro translational activity from the MMTV 5'-UTR was resistant to the addition of m(7)GpppG cap-analog and cleavage of eIF4G by foot-and-mouth disease virus (FMDV) L-protease. IRES activity was also demonstrated in the Xenopus laevis oocyte by micro-injection of capped and polyadenylated bicistronic RNAs harboring the MMTV-5'-UTR. Finally, transfection assays showed that the MMTV-IRES exhibits cell type-dependent translational activity, suggesting a requirement for as yet unidentified cellular factors for its optimal function.
dc.description.funderComision Nacional de Investigacion Cientifica y Tecnologica, Gobierno de Chile, CONICYT
dc.description.funderMIFAB Institute
dc.description.funderMECE(2) SUP-UNAB Doctoral Fellowship
dc.fechaingreso.objetodigital2024-04-26
dc.format.extent15 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1093/nar/gkp890
dc.identifier.eissn1362-4962
dc.identifier.issn0305-1048
dc.identifier.pubmedidMEDLINE:19889724
dc.identifier.urihttps://doi.org/10.1093/nar/gkp890
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79806
dc.identifier.wosidWOS:000274088800030
dc.information.autorucCiencias Biológicas;Huidobro-Toro JP;S/I;98862
dc.information.autorucCiencias Biológicas;Lowy F;S/I;149766
dc.information.autorucMedicina;López-Lastra M;S/I;84823
dc.information.autorucCiencias Biológicas;Ramdohr P;S/I;119722
dc.information.autorucCiencias Biológicas;Tapia K;S/I;119823
dc.information.autorucMedicina;Valiente-Echeverría F;S/I;1006402
dc.information.autorucMedicina;Vallejos M;S/I;132558
dc.issue.numero2
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final632
dc.pagina.inicio618
dc.publisherOXFORD UNIV PRESS
dc.revistaNUCLEIC ACIDS RESEARCH
dc.rightsacceso abierto
dc.subjectINTERNAL RIBOSOME ENTRY
dc.subjectC-MYC-IRES
dc.subjectPROTEIN-SYNTHESIS
dc.subjectEUKARYOTIC TRANSLATION
dc.subjectHIV-1 PROTEASE
dc.subjectIN-VITRO
dc.subjectPROTEOLYTIC CLEAVAGE
dc.subjectPROMOTES TRANSLATION
dc.subjectBINDING-PROTEIN
dc.subjectFACTOR 4G
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe 5'-untranslated region of the mouse mammary tumor virus mRNA exhibits cap-independent translation initiation
dc.typeartículo
dc.volumen38
sipa.codpersvinculados98862
sipa.codpersvinculados149766
sipa.codpersvinculados84823
sipa.codpersvinculados119722
sipa.codpersvinculados119823
sipa.codpersvinculados1006402
sipa.codpersvinculados132558
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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