Enhanced Glutathione Content Allows the In Vivo Synthesis of Fluorescent CdTe Nanoparticles by Escherichia coli

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dc.contributor.authorMonras, Juan P.
dc.contributor.authorDiaz, Victor
dc.contributor.authorBravo, Denisse
dc.contributor.authorMontes, Rebecca A.
dc.contributor.authorChasteen, Thomas G.
dc.contributor.authorOsorio Roman, Igor O.
dc.contributor.authorVasquez, Claudio C.
dc.contributor.authorPerez Donoso, Jose M.
dc.date.accessioned2024-01-10T12:38:44Z
dc.date.available2024-01-10T12:38:44Z
dc.date.issued2012
dc.description.abstractThe vast application of fluorescent semiconductor nanoparticles (NPs) or quantum dots (QDs) has prompted the development of new, cheap and safer methods that allow generating QDs with improved biocompatibility. In this context, green or biological QDs production represents a still unexplored area. This work reports the intracellular CdTe QDs biosynthesis in bacteria. Escherichia coli overexpressing the gshA gene, involved in glutathione (GSH) biosynthesis, was used to produce CdTe QDs. Cells exhibited higher reduced thiols, GSH and Cd/Te contents that allow generating fluorescent intracellular NP-like structures when exposed to CdCl2 and K2TeO3. Fluorescence microscopy revealed that QDs-producing cells accumulate defined structures of various colors, suggesting the production of differently-sized NPs. Purified fluorescent NPs exhibited structural and spectroscopic properties characteristic of CdTe QDs, as size and absorption/emission spectra. Elemental analysis confirmed that biosynthesized QDs were formed by Cd and Te with Cd/Te ratios expected for CdTe QDs. Finally, fluorescent properties of QDs-producing cells, such as color and intensity, were improved by temperature control and the use of reducing buffers.
dc.description.funderFONDECYT (Fondo Nacional de Investigacion Cientifica y Tecnologica)
dc.description.funderDicyt-USACH (Direccion de Investigacion-Universidad de Santiago de Chile)
dc.description.funderIFS (International Foundation for Science, Sweden)
dc.description.funderWelch Foundation
dc.description.funderCONICYT (Comision Nacional de Ciencia y Tecnologia)
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pone.0048657
dc.identifier.issn1932-6203
dc.identifier.pubmedidMEDLINE:23185270
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0048657
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77090
dc.identifier.wosidWOS:000311821000018
dc.information.autorucQuímica;Osorio I;S/I;207713
dc.issue.numero11
dc.language.isoen
dc.nota.accesoSin adjunto
dc.publisherPUBLIC LIBRARY SCIENCE
dc.revistaPLOS ONE
dc.rightsregistro bibliográfico
dc.subjectONE-POT SYNTHESIS
dc.subjectQUANTUM DOTS
dc.subjectMAGNETOTACTIC BACTERIA
dc.subjectMETAL NANOPARTICLES
dc.subjectMICROBIAL SYNTHESIS
dc.subjectCDS NANOCRYSTALS
dc.subjectBIOSYNTHESIS
dc.subjectTELLURITE
dc.subjectCELL
dc.subjectLUMINESCENT
dc.titleEnhanced Glutathione Content Allows the In Vivo Synthesis of Fluorescent CdTe Nanoparticles by Escherichia coli
dc.typeartículo
dc.volumen7
sipa.codpersvinculados207713
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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