Microbial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses

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dc.contributor.authorDiGiulio, Daniel B.
dc.contributor.authorGervasi, Maria Teresa
dc.contributor.authorRomero, Roberto
dc.contributor.authorVaisbuch, Edi
dc.contributor.authorMazaki Tovi, Shali
dc.contributor.authorKusanovic, Juan Pedro
dc.contributor.authorSeok, Kimberley S.
dc.contributor.authorGomez, Ricardo
dc.contributor.authorMittal, Pooja
dc.contributor.authorGotsch, Francesca
dc.contributor.authorChaiworapongsa, Tinnakorn
dc.contributor.authorOyarzun, Enrique
dc.contributor.authorKim, Chong Jai
dc.contributor.authorRelman, David A.
dc.date.accessioned2024-01-10T13:50:28Z
dc.date.available2024-01-10T13:50:28Z
dc.date.issued2010
dc.description.abstractObjective: Microbial invasion of the amniotic cavity (MIAC) has been detected in women with preterm labor, preterm prelabor rupture of membranes (PROM), and in patients at term with PROM or in spontaneous labor. Intrauterine infection is recognized as a potential cause of fetal growth restriction; yet, the frequency of MIAC in pregnancies with small-for-gestational-age (SGA) fetuses is unknown. The aim of this study was to determine the frequency, diversity and relative abundance of microbes in amniotic fluid (AF) of women with an SGA neonate using a combination of culture and molecular methods.
dc.description.abstractMethod: AF from 52 subjects with an SGA neonate was analyzed with both cultivation and molecular methods in a retrospective cohort study. Broad-range and group-specific PCR assays targeted small subunit rDNA, or other gene sequences, from bacteria, fungi and archaea.
dc.description.abstractResults of microbiologic studies were correlated with indices of the host inflammatory response. Results: 1) All AF samples (n=52) were negative for microorganisms based on cultivation techniques, whereas 6% (3/52) were positive based on PCR; and 2) intra-amniotic inflammation was detected in one of the three patients with a positive PCR result, as compared with three patients (6.1%) of the 49 with both a negative culture and a negative PCR (P=0.2).
dc.description.abstractConclusion: MIAC is detected by PCR in some patients with an SGA fetus who were not in labor at the time of AF collection.
dc.description.funderEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, DHHS
dc.description.funderMarch of Dimes Foundation
dc.description.funderNIH
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT
dc.description.funderOFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1515/JPM.2010.076
dc.identifier.eissn1619-3997
dc.identifier.issn0300-5577
dc.identifier.pubmedidMEDLINE:20482466
dc.identifier.urihttps://doi.org/10.1515/JPM.2010.076
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79533
dc.identifier.wosidWOS:000281566600008
dc.information.autorucMedicina;Gomez R ;S/I;80926
dc.information.autorucMedicina;Oyarzun E;S/I;54261
dc.issue.numero5
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final502
dc.pagina.inicio495
dc.publisherWALTER DE GRUYTER GMBH
dc.revistaJOURNAL OF PERINATAL MEDICINE
dc.rightsregistro bibliográfico
dc.subject16S rRNA
dc.subjectchorioamnionitis
dc.subjectcytokines
dc.subjectFIRS
dc.subjectIL-6
dc.subjectintra-amniotic infection
dc.subjectintra-amniotic inflammation
dc.subjectmolecular microbiology
dc.subjectPCR
dc.subjectpregnancy
dc.subjectSGA
dc.subjectFETAL-GROWTH RESTRICTION
dc.subjectNECROSIS-FACTOR-ALPHA
dc.subjectPORPHYROMONAS-GINGIVALIS INFECTION
dc.subjectPRETERM PREMATURE RUPTURE
dc.subjectPOLYMERASE-CHAIN-REACTION
dc.subjectEPSTEIN-BARR-VIRUS
dc.subjectREAL-TIME PCR
dc.subjectCLINICAL-SIGNIFICANCE
dc.subjectINTRAAMNIOTIC INFECTION
dc.subjectUREAPLASMA-UREALYTICUM
dc.subject.ods03 Good Health and Well-being
dc.subject.ods05 Gender Equality
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa05 Igualdad de género
dc.titleMicrobial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses
dc.typeartículo
dc.volumen38
sipa.codpersvinculados80926
sipa.codpersvinculados54261
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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