Increased activity in the biliary Con A-binding fraction accounts for the difference in crystallization behavior in bile from Chilean gallstone patients compared with Dutch gallstone patients

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dc.contributor.authorMiquel, JF
dc.contributor.authorvan der Putten, J
dc.contributor.authorPimentel, F
dc.contributor.authorMok, KS
dc.contributor.authorGroen, AK
dc.date.accessioned2024-01-10T13:49:21Z
dc.date.available2024-01-10T13:49:21Z
dc.date.issued2001
dc.description.abstractChile has one of the highest prevalences of cholesterol gallstone disease in the world. Recent data indicate that this is partly caused by genetic (Indian) factors. However, the causal factors inducing increased gallstone formation have not been elucidated. The aim of this study was to compare biliary composition and cholesterol crystallization in bile from patients of high and moderate risk areas (Chile and The Netherlands) for gallstone disease. Bile was sampled at cholecystectomy from 30 Chilean and 26 Dutch gallstone patients. The Con A-binding fraction (CABF) was extracted from fresh native bile samples by incubation with Con A-sepharose. Reconstitution of the CABF to the Con A-extracted native bile induced almost full recovery of crystallization confirming the validity of this technique. There was no difference between the two groups regarding sex and age. Chilean bile nucleated significantly faster (3.5 +/- 0.6 vs. 7.9 +/- 1.5 days) despite the fact that Dutch bile had a significantly higher cholesterol saturation index (CSI) (1.6 vs. 1.2, P = .01). The total lipid content was not different. Chilean bile contained more total protein (5 vs. 2.9 mg/mL, P = .008), IgG, IgM, Haptoglobin and alpha -1-acid glycoprotein were not different between the two groups. IgA, though, was significantly higher in the Chilean samples (0.44 vs. 0.19 mg/mL, P <.001). Extraction of CABF increased crystal observation time (COT) and decreased crystal growth in both groups. However, the effects were much more pronounced in the Chilean samples. Compared with Dutch bile, Chilean bile crystallizes much faster despite a lower CSI. Chilean bile contains an increased content of Con A-binding nucleation promoting activity.
dc.fechaingreso.objetodigital2024-04-25
dc.format.extent5 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1053/jhep.2001.21550
dc.identifier.issn0270-9139
dc.identifier.pubmedidMEDLINE:11172333
dc.identifier.urihttps://doi.org/10.1053/jhep.2001.21550
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79444
dc.identifier.wosidWOS:000166705800002
dc.information.autorucMedicina;Miquel J;S/I;72216
dc.issue.numero2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final332
dc.pagina.inicio328
dc.publisherW B SAUNDERS CO
dc.revistaHEPATOLOGY
dc.rightsacceso restringido
dc.subjectHUMAN GALLBLADDER BILE
dc.subjectCHOLESTEROL CRYSTALLIZATION
dc.subjectCRYSTAL-FORMATION
dc.subjectNUCLEATION TIME
dc.subjectPROTEINS
dc.subjectAPOLIPOPROTEINS
dc.subjectGLYCOPROTEINS
dc.subjectSATURATION
dc.subjectSOLUBILITY
dc.subjectDISEASE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIncreased activity in the biliary Con A-binding fraction accounts for the difference in crystallization behavior in bile from Chilean gallstone patients compared with Dutch gallstone patients
dc.typeartículo
dc.volumen33
sipa.codpersvinculados72216
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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