Platelet-Poor and Platelet-Rich Plasma Stimulate Bone Lineage Differentiation in Periodontal Ligament Stem Cells

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dc.contributor.authorMartinez, C. E.
dc.contributor.authorGonzález Bombardiere, Sergio
dc.contributor.authorPalma, V.
dc.contributor.authorSmith, P. C.
dc.date.accessioned2019-10-16T20:16:11Z
dc.date.available2019-10-16T20:16:11Z
dc.date.issued2016
dc.description.abstractBackground: Plasma-derived fractions have been used as an autologous source of growth factors; however, limited knowledge concerning their biologic effects has hampered their clinical application. In this study, the authors analyze the content and specific effect of both platelet-rich plasma (PRP) and platelet-poor plasma (PPP) on osteoblastic differentiation using primary cultures of human periodontal ligament stem cells (HPLSCs). Methods: The authors evaluated the growth factor content of PRP and PPP using a proteome profiler array and enzyme-linked immunosorbent assay. HPLSCs were characterized by flow cytometry and differentiation assays. The effect of PRP and PPP on HPLSC bone differentiation was analyzed by quantifying calcium deposition after 14 and 21 days of treatment. Results: Albeit at different concentrations, the two fractions had similar profiles of growth factors, the most representative being platelet-derived growth factor (PDGF) isoforms (PDGF-AA, -BB, and -AB), insulin-like growth factor binding protein (IGFBP)-2, and IGFBP-6. Both formulations exerted a comparable stimulus on osteoblastic differentiation even at low doses (2.5%), increasing calcium deposits in HPLSCs. Conclusions: PRP and PPP showed a similar protein profile and exerted comparable effects on bone differentiation. Further studies are needed to characterize and compare the effects of PPP and PRP on bone healing in vivo.
dc.format.extent9 páginas
dc.fuente.origenFacultad de Medicina
dc.identifier.doi10.1902/jop.2015.150360
dc.identifier.issn0022-3492
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26570
dc.issue.numeroNo. 2
dc.language.isoen
dc.pagina.finalE26
dc.pagina.inicioE18
dc.revistaJournal of Periodontologyes_ES
dc.subjectCalcification, physiologices_ES
dc.subjectcell differentiationes_ES
dc.subjectmesenchymal stromales_ES
dc.subjectcellses_ES
dc.subjectperiodontal ligamentes_ES
dc.subjectplatelet-rich plasmaes_ES
dc.subjectregenerationes_ES
dc.subjectgrowth-factorses_ES
dc.subjectgingival fibroblastses_ES
dc.subjecttissue regenerationes_ES
dc.subjectrhpdgf-bbes_ES
dc.subjectin-vivoes_ES
dc.subjecttherapyes_ES
dc.subjectrepaires_ES
dc.subjecttechnologieses_ES
dc.subjectmigrationes_ES
dc.subjectcriteriaes_ES
dc.subjectDentistry, Oral Surgery & Medicinees_ES
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titlePlatelet-Poor and Platelet-Rich Plasma Stimulate Bone Lineage Differentiation in Periodontal Ligament Stem Cellses_ES
dc.typeartículo
dc.volumenVol. 87
sipa.codpersvinculados1008613
sipa.codpersvinculados99856
sipa.codpersvinculados1006488
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