Morphological and molecular identification of canine filariae in a semi-rural district of the Metropolitan Region in Chile

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dc.contributor.authorLopez, Javier
dc.contributor.authorValiente Echeverria, Fernando
dc.contributor.authorCarrasco, Marcela
dc.contributor.authorMercado, Ruben
dc.contributor.authorAbarca, Katia
dc.date.accessioned2024-01-10T13:50:45Z
dc.date.available2024-01-10T13:50:45Z
dc.date.issued2012
dc.description.abstractIntroduction: To date, there has been no definitive confirmation of the presence of zoonotic dirofilariasis in dogs in Chile. Objectives: To study the presence of dirofilarias in blood samples from dogs collected in a semi-rural district near Santiago and to compare their frequency in dogs with and without dermatological manifestations. Methods: We examined 100 blood samples for dog filariae infections using microscopic methods (modified Knott technique). 50 dogs presented dermatological symptoms or signs compatible with filarial infections and 50 were asymptomatic. ITS-2 and 12s rDNA gene amplification by PCR and sequencing were performed in samples microscopically positive for microfilariae. Results. We observed microfilariae in 22 dogs (22%). Of these, 16/50 (32%) were symptomatic and 6/50 (12%) were asymptomatic (p = 0.02). Morphologically, the majority of microfilariae were similar to Dirofilaria repens, although many had a bigger size than previously described. Nucleotide sequencing of the amplified genes showed no more than 95% homology with the D. repens sequences available for comparison. D. reconditum and D. dracunculoides infections were also identified. Conclusions: These features might indicate the presence of new species of Dirofilaria or a D. repens close related variant in Chile.
dc.fechaingreso.objetodigital2024-05-30
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.identifier.doi10.4067/S0716-10182012000300006
dc.identifier.issn0716-1018
dc.identifier.pubmedidMEDLINE:23096462
dc.identifier.urihttps://doi.org/10.4067/S0716-10182012000300006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79553
dc.identifier.wosidWOS:000305923600006
dc.information.autorucMedicina;Abarca K;S/I;70281
dc.information.autorucMedicina;Valiente F ;S/I;1006402
dc.issue.numero3
dc.language.isoes
dc.nota.accesoContenido completo
dc.pagina.final289
dc.pagina.inicio284
dc.publisherSOC CHILENA INFECTOLOGIA
dc.revistaREVISTA CHILENA DE INFECTOLOGIA
dc.rightsacceso abierto
dc.subjectCanine filariasis
dc.subjectmicrofilariae
dc.subjectDirofilaria repens
dc.subjectzoonotic dirofilariasis
dc.subjectSOUTH-AMERICA
dc.subjectDOGS
dc.subjectDIROFILARIASIS
dc.subjectCATS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMorphological and molecular identification of canine filariae in a semi-rural district of the Metropolitan Region in Chile
dc.typeartículo
dc.volumen29
sipa.codpersvinculados70281
sipa.codpersvinculados1006402
sipa.indexWOS
sipa.indexScielo
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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