Sex-dependent differences on the impact of anti-inflammatory treatment in the progression of coronary artery disease in a murine model of lethal ischemic heart disease induced by diet

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dc.catalogadorgjm
dc.contributor.advisorAndía Kohnenkampf, Marcelo Edgardo
dc.contributor.advisorGonzález, Leticia
dc.contributor.authorParra Núñez, Laura Macarena
dc.contributor.otherPontificia Universidad Católica de Chile. Facultad de Medicina
dc.date.accessioned2023-11-02T19:24:33Z
dc.date.issued2023
dc.date.updated2023-11-02T18:55:26Z
dc.descriptionTesis (Magíster en Investigación en Ciencias de la Salud)--Pontificia Universidad Católica de Chile, 2023.
dc.description.abstractCardiovascular risk differs significantly between adult men and women. Therefore, it is expected that different treatments may affect both groups differently. We aim to compare sexdependent differences on survival and systemic inflammation in response to anti-inflammatory treatment using a diet-induced myocardial infarction mouse model. Method. Male and female SR-B1−/−ApoER61h/h mice, aged 2-3 months, were randomly assigned into two groups: Control (HFD-Control) and minocycline (HFD-MIN). Atherosclerosis was induced by feeding an atherogenic diet (15% fat, 1.25% cholesterol, 0.5% cholate). Minocycline was administered in the drinking water at a dose of 0.05 mg/mL. Female mice had a slightly better survival than male mice when fed an HFD (p=0.12). Minocycline improved survival in male by 35% (p=0.006) and by 33% in female p=0.01), without affecting total cholesterol levels. Male mice fed with HFD tended to have higher IL-6 levels than female mice (p=0.08). Minocycline significantly reduced IL-6 levels (p=0.04) and Ly6Chigh (p=0.006) and increased the Ly6Clow subset (p=0.006). Male and female fed with HFD clustered in different groups by analyzing inflammatory parameters by PCA; however, after minocycline intervention, were indistinguishable. High fat diet decreased survival and caused early death in this animal model, however, females had slightly better survival than male mice. Minocycline treatment improved survival in both groups although it did not affect their cholesterol levels. Males showed higher inflammatory serum biomarkers than females, and minocycline treatment showed a higher impact on systemic anti-inflammation in male mice than female, by reducing plasma IL-6 levels and shifting toward a more "reparative" phenotype on circulating monocyte subsets.
dc.description.version2023-11-04
dc.fechaingreso.objetodigital2023-11-02
dc.format.extent19 páginas sin numerar
dc.fuente.origenAutoarchivo
dc.identifier.doi10.7764/tesisUC/MED/75224
dc.identifier.urihttp://doi.org/10.7764/tesisUC/MED/75224
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75224
dc.information.autorucEscuela de medicina; Andía Kohnenkampf, Marcelo Edgardo; 0000-0002-1251-5832; 90691
dc.information.autorucEscuela de Medicina; González, Leticia; 0000-0002-0589-6884; 1061572
dc.information.autorucEscuela de Medicina; Parra Núñez, Laura Macarena; S/I; 215796
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subjectAtherosclerosis
dc.subjectGender
dc.subjectInflammation
dc.subjectMonocytes/macrophages
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleSex-dependent differences on the impact of anti-inflammatory treatment in the progression of coronary artery disease in a murine model of lethal ischemic heart disease induced by diet
dc.typetesis de maestría
sipa.codpersvinculados90691
sipa.codpersvinculados1061572
sipa.codpersvinculados215796
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