A PPARs cross-talk concertedly commits C6 glioma cells to oligodendrocytes and induces enzymes involved in myelin synthesis

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dc.contributor.authorLeisewitz, Andrea V.
dc.contributor.authorUrrutia, Carolina R.
dc.contributor.authorMartinez, Gabriela R.
dc.contributor.authorLoyola, Gloria
dc.contributor.authorBronfman, Miguel
dc.date.accessioned2024-01-10T12:04:17Z
dc.date.available2024-01-10T12:04:17Z
dc.date.issued2008
dc.description.abstractPeroxisome proliferator activated receptors (PPARs, alpha, beta/delta, gamma) control lipid homeostasis and differentiation in various tissues and tumor cells. PPAR beta and PPAR gamma increase oligodendrocyte maturation in glial mixed populations and spinal cord oligodendrocytes, respectively, and PPAR beta is known to modulate the activity of other PPARs. To assess a possible interaction between PPARs in glial cell differentiation we used the undifferentiated C6 glioma cell line as model. These cells express all three PPARs, but only PPAR gamma shows transcriptional activity in agonist-based reporter gene assay. Agonist-activated PPAR gamma up-regulates oligodendrocyte markers, down-regulates an astrocyte marker, and increases alkyl-dihydroxyacetone phosphate synthase, enzyme involved in the synthesis of myelin-rich plasmalogens. Similar effects are induced in PPAR gamma overexpressing cells, which in addition show PPAR beta up-regulation. PPAR beta or PPAR alpha agonists show no effect. Nevertheless, PPAR beta overexpression up-regulates PPAR gamma and commits C6 cells to oligodendrocytes: effect that is abrogated by a PPAR gamma antagonist or PPAR gamma interference RNA. Moreover, PPAR beta overexpression also induces PPAR alpha and its target genes, including acyl-CoA oxidase, enzyme involved in very long chain fatty acid recycling, and in the synthesis of myelin components such as docosahexaenoic acid. These results indicate for the first time, that PPARs concertedly cooperate in C6 glioma cell differentiation to oligodendrocytes. Further, they suggest that active PPAR beta might be essential for increasing oligodendrocyte distinctive markers and enzymes required for myelin synthesis in C6 glioma cells through up-regulation of PPAR gamma and PPAR alpha.
dc.fechaingreso.objetodigital2024-04-27
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/jcp.21509
dc.identifier.eissn1097-4652
dc.identifier.issn0021-9541
dc.identifier.pubmedidMEDLINE:18543250
dc.identifier.urihttps://doi.org/10.1002/jcp.21509
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75748
dc.identifier.wosidWOS:000259721800010
dc.information.autorucCiencias Biológicas;Bronfman M;S/I;98819
dc.information.autorucCiencias Biológicas;Leisewitz A;S/I;94860
dc.information.autorucCiencias Biológicas;Loyola G;S/I;75511
dc.information.autorucCiencias Biológicas;Martínez B;S/I;127295
dc.information.autorucCiencias Biológicas;Urrutia C;S/I;132616
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final376
dc.pagina.inicio367
dc.publisherWILEY
dc.revistaJOURNAL OF CELLULAR PHYSIOLOGY
dc.rightsacceso restringido
dc.subjectACTIVATED-RECEPTOR-GAMMA
dc.subjectMESSENGER-RNAS
dc.subjectTARGETED DISRUPTION
dc.subjectPEROXISOME
dc.subjectGROWTH
dc.subjectRAT
dc.subjectEXPRESSION
dc.subjectALPHA
dc.subjectDELTA
dc.subjectBETA
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA PPARs cross-talk concertedly commits C6 glioma cells to oligodendrocytes and induces enzymes involved in myelin synthesis
dc.typeartículo
dc.volumen217
sipa.codpersvinculados98819
sipa.codpersvinculados94860
sipa.codpersvinculados75511
sipa.codpersvinculados127295
sipa.codpersvinculados132616
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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