CHRONIC FLUOXETINE TREATMENT INDUCES STRUCTURAL PLASTICITY AND SELECTIVE CHANGES IN GLUTAMATE RECEPTOR SUBUNITS IN THE RAT CEREBRAL CORTEX

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dc.contributor.authorAmpuero, E.
dc.contributor.authorRubio, F. J.
dc.contributor.authorFalcon, R.
dc.contributor.authorSandoval, M.
dc.contributor.authorDiaz Veliz, G.
dc.contributor.authorGonzalez, R. E.
dc.contributor.authorEarle, N.
dc.contributor.authorDagnino Subiabre, A.
dc.contributor.authorAboitiz, F.
dc.contributor.authorOrrego, F.
dc.contributor.authorWyneken, U.
dc.date.accessioned2024-01-10T13:11:43Z
dc.date.available2024-01-10T13:11:43Z
dc.date.issued2010
dc.description.abstractIt has been postulated that chronic administration of antidepressant drugs induces delayed structural and molecular adaptations at glutamatergic forebrain synapses that might underlie mood improvement. To gain further insight into these changes in the cerebral cortex, rats were treated with fluoxetine (flx) for 4 weeks. These animals showed decreased anxiety and learned helplessness. N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor subunit levels (NR1, NR2A, NR2B, GluR1 and GluR2) were analysed in the forebrain by both western blot of homogenates and immunohistochemistry. Both methods demonstrated an upregulation of NR2A, GluR1 and GluR2 that was especially significant in the retrosplenial granular b cortex (RSGb). However, when analysing subunit content in postsynaptic densities and synaptic membranes, we found increases of NR2A and GluR2 but not GluR1. Instead, GluR1 was augmented in a microsomal fraction containing intracellular membranes. NR1 and GluR2 were co-immunoprecipitated from postsynaptic densities and synaptic membranes. In the immunoprecipitates, NR2A was increased while GluR1 was decreased supporting a change in receptor stoichiometry. The changes of subunit levels were associated with an upregulation of dendritic spine density and of large, mushroom-type spines. These molecular and structural adaptations might be involved in neuronal network stabilization following long-term fix treatment. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
dc.description.funderProyecto Anillo
dc.fechaingreso.objetodigital03-04-2024
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.neuroscience.2010.04.035
dc.identifier.eissn1873-7544
dc.identifier.issn0306-4522
dc.identifier.pubmedidMEDLINE:20417256
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2010.04.035
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78087
dc.identifier.wosidWOS:000279579200009
dc.information.autorucMedicina;Aboitiz F;S/I;100165
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final108
dc.pagina.inicio98
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.revistaNEUROSCIENCE
dc.rightsacceso restringido
dc.subjectantidepressants
dc.subjectglutamate
dc.subjectionotropic receptors
dc.subjectdendritic spines
dc.subjectcerebral cortex
dc.subjectAMPA RECEPTORS
dc.subjectSYNAPTIC PLASTICITY
dc.subjectDENDRITIC SPINES
dc.subjectINCREASES EXPRESSION
dc.subjectPREFRONTAL CORTEX
dc.subjectFIRING RATE
dc.subjectNMDA
dc.subjectSTRESS
dc.subjectHIPPOCAMPUS
dc.subjectGLUR2
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCHRONIC FLUOXETINE TREATMENT INDUCES STRUCTURAL PLASTICITY AND SELECTIVE CHANGES IN GLUTAMATE RECEPTOR SUBUNITS IN THE RAT CEREBRAL CORTEX
dc.typeartículo
dc.volumen169
sipa.codpersvinculados100165
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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