Signaling pathways involved in the repression of root nitrate uptake by nitrate in Arabidopsis thaliana

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dc.catalogadorpva
dc.contributor.authorValentin Chaput
dc.contributor.authorJianfu Li
dc.contributor.authorDavid Séré
dc.contributor.authorPascal Tillard
dc.contributor.authorCécile Fizames
dc.contributor.authorTomas Moyano
dc.contributor.authorKaijing Zuo
dc.contributor.authorAntoine Martin
dc.contributor.authorRodrigo A. Gutiérrez
dc.contributor.authorAlain Gojon
dc.contributor.authorLaurence Lejay
dc.date.accessioned2024-03-04T15:19:37Z
dc.date.available2024-03-04T15:19:37Z
dc.date.issued2022
dc.description.abstractIn Arabidopsis thaliana, root high-affinity nitrate (NO3-) uptake depends mainly on NRT2.1, 2.4 and 2.5, which are repressed by high NO3- supply at the transcript level. For NRT2.1, this regulation is due to the action of (i) feedback downregulation by N metabolites and (ii) repression by NO3- itself mediated by the transceptor NRT1.1(NPF6.3). However, for NRT2.4 and NRT2.5 the signaling pathway(s) remain unknown along with the molecular elements involved. Here we show that unlike NRT2.1, NRT2.4 and NRT2.5 are not induced in a NO3- reductase mutant but are strongly upregulated following replacement of NO3- by ammonium (NH4+) as the N source. Moreover, increasing NO3- concentration in a mixed nutrient solution with constant NH4+ concentration results in a gradual repression of NRT2.4 and NRT2.5, which is suppressed in a nrt1.1 mutant. This indicates that NRT2.4 and NRT2.5 are subjected to repression by NRT1.1-mediated NO3- sensing, and not to feedback repression by reduced N metabolites. We further show that key regulators of NRT2s transporters, such as HHO1, HRS1, PP2C, LBD39, BT1 and BT2, are also regulated by NRT1.1-mediated NO3- sensing, and that several are involved in NO3- repression of NRT2.1, 2.4 and 2.5. Finally, we provide evidence that it is the phosphorylated form of NRT1.1 at the T101 residue, which is most active in triggering the NRT1.1-mediated NO3- regulation of all these genes. Altogether, these data led to propose a regulatory model for high-affinity NO3- uptake in Arabidopsis, highlighting several NO3- transduction cascades downstream the phosphorylated form of the NRT1.1 transceptor. One sentence summary Characterisation and identification of molecular elements involved in the signaling pathways repressing NRT2s transporters and root nitrate uptake in response to nitrate.
dc.fechaingreso.objetodigital2024-02-26
dc.format.extent33 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1101/2022.10.07.511332
dc.identifier.urihttps://doi.org/10.1101/2022.10.07.511332
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/82434
dc.language.isoen
dc.nota.accesoContenido parcial
dc.revistabioRxiv
dc.rightsacceso restringido
dc.subjectNO3 - acquisition
dc.subjectNRT2s
dc.subjectBTB genes
dc.subjectNIGTs
dc.subjectLBDs
dc.subjectNO3 - signaling
dc.subjectNRT1.1
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleSignaling pathways involved in the repression of root nitrate uptake by nitrate in Arabidopsis thaliana
dc.typepreprint
sipa.codpersvinculados86782
sipa.trazabilidadORCID;2024-01-15
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