Nitric oxide synthesis requires activity of the cationic and neutral amino acid transport system y(+)L in human umbilical vein endothelium
dc.contributor.author | Arancibia Garavilla, Y | |
dc.contributor.author | Toledo, F | |
dc.contributor.author | Casanello, P | |
dc.contributor.author | Sobrevia, L | |
dc.date.accessioned | 2024-01-10T12:40:51Z | |
dc.date.available | 2024-01-10T12:40:51Z | |
dc.date.issued | 2003 | |
dc.description.abstract | L-Arginine transport is mediated by the cationic/neutral amino acid transport system y(+)L and cationic amino acid transporters y(+)/CATS in human umbilical vein endothelial cells (HUVECs). System y(+)/CATS activity may be rate-limiting for nitric oxide (NO) synthesis, but no reports have demonstrated system y(+)L involvement in NO synthesis in endothelium. We investigated the role of system y(+)L in NO synthesis in HUVECs. Transport of 1.5 muM L-arginine was inhibited (P < 0.05) by L-lysine (K-i, 1.4 muM), L-leucine (K-i,1.8 mum) and L-phenylalanine (K-i, 4.1 muM), but was unaltered (P > 0.05) by L-alanine or L-cysteine. The system y(+)/CATS inhibitor, N-ethylmaleimide (NEM), did not alter 1.5 muM L-arginine transport, but inhibited (92 +/- 3 %) 100 muM L-arginine transport. L-Arginine transport in the presence of NEM was saturable (V-max, 0.37 +/- 0.02 pmol (mug protein)(-1) min(-1); K-m, 1.5 +/- 0.3 muM) and competitively inhibited by L-leucine in the presence of Na+ (V-max, 0.49 +/- 0.06 pmol (mug protein)(-1) min(-1); K-m, 6.5 +/- 0.9 muM). HUVECs express SLC3A2/4F2hc, SLC7A7/4F2-lc2 and SLC7A6/4F2-lc3 genes encoding for the high-affinity transport system y(+)L. N-G-Nitro-L-arginine methyl ester and L-leucine, but not NEM, inhibited NO synthesis in medium containing 1.5 muM L-arginine. Cells exposed to 25 MM D-glucose (24 h) exhibited reduced system y(+)L activity (V-max, 0.15 +/- 0.008 pmol (mug protein)(-1) min(-1); K-m, 1.4 +/- 0.3 muM) and NO synthesis. However, 25 HIM D-glucose increased NO synthesis and L-arginine transport via system y(+). Thus, L-arginine transport through system y(+)L plays a role in NO synthesis, which could be a determining factor in pathological conditions where the endothelial L-arginine-NO pathway is altered, such as in diabetes mellitus. | |
dc.fechaingreso.objetodigital | 2024-05-14 | |
dc.format.extent | 12 páginas | |
dc.fuente.origen | WOS | |
dc.identifier.doi | 10.1113/eph8802647 | |
dc.identifier.eissn | 1469-445X | |
dc.identifier.issn | 0958-0670 | |
dc.identifier.pubmedid | MEDLINE:14603368 | |
dc.identifier.uri | https://doi.org/10.1113/eph8802647 | |
dc.identifier.uri | https://repositorio.uc.cl/handle/11534/77355 | |
dc.identifier.wosid | WOS:000186737000004 | |
dc.information.autoruc | Medicina;Sobrevia L;S/I;1002656 | |
dc.issue.numero | 6 | |
dc.language.iso | en | |
dc.nota.acceso | contenido parcial | |
dc.pagina.final | 710 | |
dc.pagina.inicio | 699 | |
dc.publisher | WILEY | |
dc.revista | EXPERIMENTAL PHYSIOLOGY | |
dc.rights | acceso restringido | |
dc.subject | L-ARGININE TRANSPORT | |
dc.subject | LYSINURIC PROTEIN INTOLERANCE | |
dc.subject | HUMAN ERYTHROCYTES | |
dc.subject | MEMBRANE-PROTEIN | |
dc.subject | PLASMA-MEMBRANE | |
dc.subject | SURFACE-ANTIGEN | |
dc.subject | HUMAN PLACENTA | |
dc.subject | D-GLUCOSE | |
dc.subject | KINASE-C | |
dc.subject | CELLS | |
dc.subject.ods | 03 Good Health and Well-being | |
dc.subject.odspa | 03 Salud y bienestar | |
dc.title | Nitric oxide synthesis requires activity of the cationic and neutral amino acid transport system y(+)L in human umbilical vein endothelium | |
dc.type | artículo | |
dc.volumen | 88 | |
sipa.codpersvinculados | 1002656 | |
sipa.index | WOS | |
sipa.index | Scopus | |
sipa.trazabilidad | Carga SIPA;09-01-2024 |