Universal loop assembly: open, efficient and cross-kingdom DNA fabrication.

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dc.article.numberysaa001
dc.catalogadorgjm
dc.contributor.authorPollak, Bernardo
dc.contributor.authorMatute Torres, Tamara Francisca
dc.contributor.authorNúñez Quijada, Isaac Natán
dc.contributor.authorCerda Rojas, Ariel
dc.contributor.authorLópez Sierra, Constanza Andrea
dc.contributor.authorVargas Torres, Valentina Isabel
dc.contributor.authorKan, Anton
dc.contributor.authorBielinski,Vincent
dc.contributor.authorDassow, Peter von
dc.contributor.authorDupont, Chris L.
dc.contributor.authorFederici, Fernán
dc.date.accessioned2023-05-19T20:25:31Z
dc.date.available2023-05-19T20:25:31Z
dc.date.issued2020
dc.description.abstractStandardized type IIS DNA assembly methods are becoming essential for biological engineering and research. These methods are becoming widespread and more accessible due to the proposition of a 'common syntax' that enables higher interoperability between DNA libraries. Currently, Golden Gate (GG)-based assembly systems, originally implemented in hostspecific vectors, are being made compatible with multiple organisms. We have recently developed the GG-based Loop assembly system for plants, which uses a small library and an intuitive strategy for hierarchical fabrication of large DNA constructs (>30 kb). Here, we describe 'universal Loop' (uLoop) assembly, a system based on Loop assembly for use in potentially any organismof choice. This design permits the use of a compact number of plasmids (two sets of four odd and even vectors), which are utilized repeatedly in alternating steps. The elements required for transformation/maintenance in target organisms are also assembled as standardized parts, enabling customization of host-specific plasmids. Decoupling of the Loop assembly logic from the host-specific propagation elements enables universal DNA assembly that retains high efficiency regardless of the final host. As a proof-of-concept, we show the engineering of multigene expression vectors in diatoms, yeast, plants and bacteria. These resources are available through the OpenMTA for unrestricted sharing and open access.
dc.fechaingreso.objetodigital2023-07-14
dc.format.extent13 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1093/synbio/ysaa001
dc.identifier.eissn2397-7000
dc.identifier.urihttps://doi.org/10.1093/synbio/ysaa001
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/67428
dc.information.autorucEscuela de Ingeniería; Matute Torres, Tamara Francisca; 0000-0002-0486-985X; 186256
dc.information.autorucEscuela de Ingeniería; Núñez Quijada, Isaac Natán; 0000-0002-4439-3745; 186208
dc.information.autorucFacultad de Ciencias Biológicas; Cerda Rojas, Ariel; S/I; 250244
dc.information.autorucFacultad de Ciencias Biológicas; López Sierra, Constanza Andrea; S/I; 215443
dc.information.autorucFacultad de Ciencias Biológicas; Vargas Torres, Valentina Isabel; S/I; 247359
dc.information.autorucFacultad de Ciencias Biológicas; Dassow, Peter von; 0000-0002-1858-1953; 1009321
dc.information.autorucFacultad de Ciencias Biológicas; Federici, Fernán; 0000-0001-9200-5383; 123357
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido completo
dc.revistaSynthetic Biology
dc.rightsacceso abierto
dc.subjectSynthetic biologyes_ES
dc.subjectOpen sourcees_ES
dc.subjectDNA assemblyes_ES
dc.subjectOpen MTAes_ES
dc.subjectFreedom to usees_ES
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleUniversal loop assembly: open, efficient and cross-kingdom DNA fabrication.es_ES
dc.typeartículo
dc.volumen5
sipa.codpersvinculados186256
sipa.codpersvinculados186208
sipa.codpersvinculados250244
sipa.codpersvinculados215443
sipa.codpersvinculados247359
sipa.codpersvinculados1009321
sipa.codpersvinculados123357
sipa.trazabilidadORCID;14-07-2023
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