Growth temperature exerts differential physiological and transcriptional responses in laboratory and wine strains of Saccharomyces cerevisiae

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dc.contributor.authorPizarro, Francisco J.
dc.contributor.authorJewett, Michael C.
dc.contributor.authorNielsen, Jens
dc.contributor.authorAgosin, Eduardo
dc.date.accessioned2024-01-10T12:05:44Z
dc.date.available2024-01-10T12:05:44Z
dc.date.issued2008
dc.description.abstractLaboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and the organoleptic properties that they confer to wine. Here, we used a two-factor design to study the responses of a standard laboratory strain, CEN.PK113-7D, and an industrial wine yeast strain, EC1118, to growth temperatures of 15 degrees C and 30 degrees C in nitrogen-limited, anaerobic, steady-state chemostat cultures. Physiological characterization revealed that the growth temperature strongly impacted the biomass yield of both strains. Moreover, we found that the wine yeast was better adapted to mobilizing resources for biomass production and that the laboratory yeast exhibited higher fermentation rates. To elucidate mechanistic differences controlling the growth temperature response and underlying adaptive mechanisms between the strains, DNA microarrays and targeted metabolome analysis were used. We identified 1,007 temperature-dependent genes and 473 strain-dependent genes. The transcriptional response was used to identify highly correlated gene expression subnetworks within yeast metabolism. We showed that temperature differences most strongly affect nitrogen metabolism and the heat shock response. A lack of stress response element-mediated gene induction, coupled with reduced trehalose levels, indicated that there was a decreased general stress response at 15 degrees C compared to that at 30 degrees C. Differential responses among strains were centered on sugar uptake, nitrogen metabolism, and expression of genes related to organoleptic properties. Our study provides global insight into how growth temperature affects differential physiological and transcriptional responses in laboratory and wine strains of S. cerevisiae.
dc.description.funderDanish Research Agency for Production and Technology
dc.description.funderChilean National Counsel for Scientific and Technologic Research
dc.description.funderOtto Monsted
dc.description.funderCenter for Microbial Biotechnology, Biocentrum-DTU
dc.description.funderDIPUC, Pontificia Universidad Catolica de Chile
dc.fechaingreso.objetodigital2024-05-14
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1128/AEM.00602-08
dc.identifier.issn0099-2240
dc.identifier.pubmedidMEDLINE:18723660
dc.identifier.urihttps://doi.org/10.1128/AEM.00602-08
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76060
dc.identifier.wosidWOS:000259985300024
dc.information.autorucIngeniería;Agosin E;S/I;99630
dc.information.autorucIngeniería;Pizarro F;S/I;9158
dc.issue.numero20
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final6368
dc.pagina.inicio6358
dc.publisherAMER SOC MICROBIOLOGY
dc.revistaAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
dc.rightsacceso restringido
dc.subjectYEAST GENE-EXPRESSION
dc.subjectCOLD-SHOCK RESPONSE
dc.subjectCHEMOSTAT CULTURES
dc.subjectALCOHOLIC FERMENTATION
dc.subjectSYSTEMS BIOLOGY
dc.subjectREGULATED GENES
dc.subjectPROTEIN
dc.subjectNITROGEN
dc.subjectGLUCOSE
dc.subjectCELLS
dc.titleGrowth temperature exerts differential physiological and transcriptional responses in laboratory and wine strains of Saccharomyces cerevisiae
dc.typeartículo
dc.volumen74
sipa.codpersvinculados99630
sipa.codpersvinculados9158
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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