nAChR-induced octopamine release mediates the effect of nicotine on a startle response in Drosophila melanogaster

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dc.catalogadorgjm
dc.contributor.authorFuenzalida Uribe, Nicolás Leonardo
dc.contributor.authorMeza Cárdenas, Rodrigo Alexis
dc.contributor.authorHoffmann, Hernán A.
dc.contributor.authorVaras Orozco, Rodrigo
dc.contributor.authorCampusano Astorga, Jorge Mauricio
dc.date.accessioned2023-01-04T12:42:50Z
dc.date.available2023-01-04T12:42:50Z
dc.date.issued2013
dc.description.abstractBiogenic amines (BAs) play a central role in the generation of complex behaviors in vertebrates and invertebrates, including the fly Drosophila melanogaster. The comparative advantages of Drosophila as a genetic model to study the contribution of BAs to behaviors stumble upon the difficulty to access the fly brain to ask relevant physiological questions. For instance, it is not known whether the activation of nicotinic acetylcholine receptors (nAChRs) induces the release of BAs in fly brain, a phenomenon associated to several behaviors in vertebrates. Here, we describe a new preparation to study the efflux of BAs in the adult fly brain by in vitro chronoamperometry. Using this preparation we show that nAChR agonists including nicotine induce a fast, transient, dose-dependent efflux of endogenous BAs, an effect mediated by -bungarotoxin-sensitive nAChRs. By using different genetic tools we demonstrate that the BA whose efflux is induced by nAChR activation is octopamine (Oct). Furthermore, we show that the impairment of a mechanically induced startle response after nicotine exposure is not observed in flies deficient in Oct transmission. Thus, our data show that the efflux of BAs in Drosophila brain is increased by nAChR activation as in vertebrates, and that then AChR-induced Oct release could have implications in a nicotine-induced behavioral response.
dc.description.funderFONDECYT
dc.fechaingreso.objetodigital2023-01-04
dc.fuente.origenHistorial Académico
dc.identifier.citationFuenzalida-Uribe N, Meza R, Hoffmann H, Varas R, Campusano J. nAChR-induced octopamine release mediates the effect of nicotine on a startle response in Drosophila melanogaster. Journal Of Neurochemistry. 2013;125(2):281-290.
dc.identifier.issn0022-3042
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1111/jnc.12161
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/66176
dc.identifier.wosidWOS:000317297700012
dc.information.autorucEscuela de medicina ; Hoffmann, Hernán A. ; 0000-0002-3142-7061 ; 179219
dc.information.autorucFacultad de ciencias biológicas ; Fuenzalida Uribe, Nicolás Leonardo ; 0000-0002-4975-7667 ; 149110
dc.information.autorucFacultad de ciencias biológicas ; Meza Cárdenas, Rodrigo Alexis ; S/I ; 149772
dc.information.autorucFacultad de ciencias biológicas ; Varas Orozco, Rodrigo ; S/I ; 2631
dc.information.autorucFacultad de ciencias biológicas ; Campusano Astorga, Jorge Mauricio ; 0000-0001-5254-8340 ; 3965
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final290
dc.pagina.inicio281
dc.revistaJournal of Neurochemistry
dc.rightsacceso abierto
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlenAChR-induced octopamine release mediates the effect of nicotine on a startle response in Drosophila melanogaster
dc.typeartículo
dc.volumen125
sipa.codpersvinculados179219
sipa.codpersvinculados149110
sipa.codpersvinculados149772
sipa.codpersvinculados2631
sipa.codpersvinculados3965
sipa.indexISI-1991-1994-1996-1997-1999-2000-2002-2003-2005-2006-2007-2008-2009-2010-2011-2012-2013-2014-2015-2016-2019
sipa.indexScopus-2010-2011-2012-2013-2014-2015
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