Test evaluation and strategy proposal to detect and to characterize carbapenemase-producing gram negative bacilli

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dc.contributor.authorMunoz, Constanza
dc.contributor.authorZumaran, Cecilia
dc.contributor.authorGonzalez, Tamara
dc.contributor.authorWozniak, Aniela
dc.contributor.authorCastillo, Claudia
dc.contributor.authorGarcia, Patricia
dc.date.accessioned2024-01-10T12:43:27Z
dc.date.available2024-01-10T12:43:27Z
dc.date.issued2017
dc.description.abstractIntroduction: The detection of carbapenemase-producing gram negative bacilli is complicated, because there are available multiple options of test. The confirmation of the enzyme by molecular characterization is not available in all laboratories in our country. Objective: To propose a fast, efficient and simple strategy to detect and confirm CPB. Materials and Methods: 39 CPB isolates and 8 non-producing were used to evaluate the phenotypic test Carba NP, CarbAcineto NP and Blue-Carba, validating the test Xpert (R) Carba-R, to be used directly with bacterial colonies with conventional PCR. Results: The sensitivity of Carba NP, CarbAcineto NP and Blue-Carba was 79,5; 87,2 y 84,6%, respectively; and specificity was 79.5; 87.2 and 84.6%, respectively. The limit of detection of Xpert (R) Carba-R was different for each carbapenemasa: 40.8 ufc/reaction to KPC and NDM and 30.6 ufc/reaction to VIM. Discussion: On isolates with decreased susceptibility to carbapenems we propose to use as screening the test CarbAcineto NP, follow by Xpert (R) Carba-R to characterize the carbapenemase and adopt specific infection control measures.
dc.fechaingreso.objetodigital2024-07-09
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.4067/s0716-10182017000400326
dc.identifier.eissn0717-6341
dc.identifier.issn0716-1018
dc.identifier.pubmedidMEDLINE:29165508
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77596
dc.identifier.wosidWOS:000411045400005
dc.information.autorucMedicina;Garcia P ;S/I;73909
dc.information.autorucMedicina;Munoz C;S/I;203285
dc.information.autorucMedicina;Wozniak A;S/I;1008612
dc.issue.numero4
dc.language.isoes
dc.nota.accesoContenido completo
dc.pagina.final332
dc.pagina.inicio326
dc.publisherSOC CHILENA INFECTOLOGIA
dc.revistaREVISTA CHILENA DE INFECTOLOGIA
dc.rightsacceso abierto
dc.subjectCarbapenemases
dc.subjectgram negative bacilli
dc.subjectXpert (R) Carba-R
dc.subjectCarba NP
dc.subjectBETA-LACTAMASE
dc.subjectKLEBSIELLA-PNEUMONIAE
dc.subjectRAPID DETECTION
dc.subjectENTEROBACTERIACEAE
dc.subjectRESISTANCE
dc.subjectALGORITHM
dc.subjectPROGRAM
dc.subjectCHILE
dc.subjectASSAY
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTest evaluation and strategy proposal to detect and to characterize carbapenemase-producing gram negative bacilli
dc.typeartículo
dc.volumen34
sipa.codpersvinculados73909
sipa.codpersvinculados203285
sipa.codpersvinculados1008612
sipa.indexWOS
sipa.indexScielo
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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