Reassessment of the toxin profile of Cylindrospermopsis raciborskii T3 and function of putative sulfotransferases in synthesis of sulfated and sulfonated PSP toxins

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dc.contributor.authorSoto Liebe, Katia
dc.contributor.authorMurillo, Alejandro A.
dc.contributor.authorKrock, Bernd
dc.contributor.authorStucken, Karina
dc.contributor.authorFuentes Valdes, Juan J.
dc.contributor.authorTrefault, Nicole
dc.contributor.authorCembella, Allan
dc.contributor.authorVasquez, Monica
dc.date.accessioned2024-01-10T14:21:31Z
dc.date.available2024-01-10T14:21:31Z
dc.date.issued2010
dc.description.abstractThe toxigenic freshwater cyanobacterium Cylindrospermopsis raciborskii T3 has been used as a model to study and elucidate the biosynthetic pathway of tetrahydropurine neurotoxins associated with paralytic shellfish poisoning (PSP). There are nevertheless several inconsistencies and contradictions in the toxin profile of this strain as published by different research groups, and claimed to include carbamoyl (SIX, NEO, GTX2/3), decarbamoyl (dcSTX), and N-sulfocarbamoyl (C1/2, 81) derivatives. Our analysis of the complete genome of another PSP toxin-producing cyanobacterium, Raphidiopsis brookii D9, which is closely related to C. raciborskii T3, resolved many issues regarding the correlation between biosynthetic pathways, corresponding genes and the T3 toxin profile. The putative sxt gene cluster in R. brookii D9 has a high synteny with the T3 sxt cluster, with 100% nucleotide identity among the shared genes. We also compared the PSP toxin profile of the strains by liquid chromatography coupled to mass spectrometry (LC-MS/MS). In contrast to published reports, our reassessment of the PSP toxin profile of T3 confirmed production of only SIX, NEO and dcNEO. We gained significant insights via correlation between specific sxt genes and their role in PSP toxin synthesis in both D9 and T3 strains. In particular, analysis of sulfotransferase functions for SxtN (N-sulfotransferase) and SxtSUL (O-sulfotransferase) enzymes allowed us to propose an extension of the PSP toxin biosynthetic pathway from SIX to the production of the derivatives GTX2/3. C1/2 and B1. This is a significantly revised view of the genetic mechanisms underlying synthesis of sulfated and sulfonated STX analogues in toxigenic cyanobacteria. (C) 2010 Elsevier Ltd. All rights reserved.
dc.description.funderChile
dc.description.funderMillennium Nucleus
dc.description.funderPrograma de Cooperacion Cientifica Internacional (PCCI) CONICYT/DFG
dc.description.funderP. Universidad Catolica de Chile
dc.description.funderAlfred Wegener Institute for Polar and Marine Research (Helmholtz Foundation)
dc.description.funderAlfred Wegener Institute as part of the Helmholtz Foundation initiative in Earth and Environment
dc.fechaingreso.objetodigital11-04-2024
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.toxicon.2010.07.022
dc.identifier.issn0041-0101
dc.identifier.pubmedidMEDLINE:20692275
dc.identifier.urihttps://doi.org/10.1016/j.toxicon.2010.07.022
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79695
dc.identifier.wosidWOS:000284664400007
dc.information.autorucCiencias Biológicas; Trefault N;S/I;134342
dc.information.autorucCiencias Biológicas;Fuentes J;S/I;142305
dc.information.autorucCiencias Biológicas;Murillo A;S/I;17856
dc.information.autorucCiencias Biológicas;Soto K;S/I;169974
dc.information.autorucCiencias Biológicas;Vasquez M ;S/I;160080
dc.issue.numero8
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final1361
dc.pagina.inicio1350
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.revistaTOXICON
dc.rightsacceso restringido
dc.subjectCylindrospermopsis raciborskii
dc.subjectRaphidiopsis brookii
dc.subjectLyngbya wollei
dc.subjectPSP toxins
dc.subjectsxt gene cluster
dc.subjectSaxitoxin
dc.subjectFRESH-WATER CYANOBACTERIUM
dc.subjectPARALYTIC SHELLFISH POISONS
dc.subjectAPHANIZOMENON-FLOS-AQUAE
dc.subjectSAXITOXIN
dc.subjectCHROMATOGRAPHY
dc.subjectANALOGS
dc.subjectGENES
dc.subject.ods06 Clean Water and Sanitation
dc.subject.ods15 Life on Land
dc.subject.ods13 Climate Action
dc.subject.odspa06 Agua limpia y saneamiento
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.subject.odspa13 Acción por el clima
dc.titleReassessment of the toxin profile of Cylindrospermopsis raciborskii T3 and function of putative sulfotransferases in synthesis of sulfated and sulfonated PSP toxins
dc.typeartículo
dc.volumen56
sipa.codpersvinculados134342
sipa.codpersvinculados142305
sipa.codpersvinculados17856
sipa.codpersvinculados169974
sipa.codpersvinculados160080
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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