Isolation and molecular characterization of MYB60 in Solanum lycopersicum

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dc.catalogadorgjm
dc.contributor.authorRodríguez Hoces de la Guardia, Amparo Cecilia
dc.contributor.authorUgalde, María Beatriz
dc.contributor.authorLobos Díaz, Valeria Rocío
dc.contributor.authorLucina Romero-Romero, Jesús
dc.contributor.authorMeyer Regueiro, Carlos José
dc.contributor.authorInostroza Blancheteau, Claudio
dc.contributor.authorReyes Diaz, Marjorie
dc.contributor.authorAquea, Felipe
dc.contributor.authorArce Johnson, Jorge Patricio
dc.date.accessioned2023-06-02T17:47:23Z
dc.date.available2023-06-02T17:47:23Z
dc.date.issued2021
dc.description.abstractStomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.
dc.fechaingreso.objetodigital2023-06-02
dc.fuente.origenORCID
dc.identifier.doi10.1007/s11033-021-06168-5
dc.identifier.eissn1573-4978
dc.identifier.issn0301-4851
dc.identifier.urihttp://doi.org/10.1007/s11033-021-06168-5
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/70504
dc.information.autorucFacultad de Ciencias Biológicas; Rodríguez Hoces de la Guardia, Amparo Cecilia; S/I; 142310
dc.information.autorucFacultad de Ciencias Biológicas; Ugalde, María Beatriz; S/I; 195505
dc.information.autorucFacultad de Ciencias Biológicas; Lobos Díaz, Valeria Rocío; S/I; 205474
dc.information.autorucFacultad de Ciencias Biológicas; Meyer Regueiro, Carlos José; S/I; 187017
dc.information.autorucFacultad de Ciencias Biológicas; Arce Johnson, Jorge Patricio; 0000-0003-3854-1286; 54718
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final1587
dc.pagina.inicio1579
dc.revistaMolecular Biology Reports
dc.rightsacceso abierto
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleIsolation and molecular characterization of MYB60 in Solanum lycopersicum
dc.typeartículo
dc.volumen48
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