Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts

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dc.catalogadorgjm
dc.contributor.authorIslam, Mohammad Mirazul
dc.contributor.authorSharifi, Roholah
dc.contributor.authorMamodaly, Shamina
dc.contributor.authorIslam, Rakibul
dc.contributor.authorNahra, Daniel
dc.contributor.authorAbusamra, Dina B.
dc.contributor.authorChuen Hui, Pui
dc.contributor.authorAdibnia, Yashar
dc.contributor.authorGoulamaly, Mehdi
dc.contributor.authorPaschalis, Eleftherios I.
dc.contributor.authorCruzat Corssen, Andrea
dc.contributor.authorKong, Jing
dc.contributor.authorNilsson, Per H.
dc.contributor.authorArgüeso, Pablo
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorChodosh, James
dc.contributor.authorDohlman, Claes H.
dc.contributor.authorGonzález-Andrades, Miguel
dc.date.accessioned2023-07-18T14:54:52Z
dc.date.available2023-07-18T14:54:52Z
dc.date.issued2019
dc.description.abstractTo address the shortcomings associated with corneal transplants, substantial efforts have been focused on developing new modalities such as xenotransplantion. Xenogeneic corneas are anatomically and biomechanically similar to the human cornea, yet their applications require prior decellularization to remove the antigenic components to avoid rejection. In the context of bringing decellularized corneas into clinical use, sterilization is a crucial step that determines the success of the transplantation. Well-standardized sterilization methods, such as gamma irradiation (GI), have been applied to decellularized porcine corneas (DPC) to avoid graft-associated infections in human recipients. However, little is known about the effect of GI on decellularized corneal xenografts. Here, we evaluated the radiation effect on the ultrastructure, optical, mechanical and biological properties of DPC. Transmission electron microscopy revealed that gamma irradiated decellularized porcine cornea (G-DPC) preserved its structural integrity. Moreover, the radiation did not reduce the optical properties of the tissue. Neither DPC nor G-DPC led to further activation of complement system compared to native porcine cornea when exposed to plasma. Although, DPC were mechanically comparable to the native tissue, GI increased the mechanical strength, tissue hydrophobicity and resistance to enzymatic degradation. Despite these changes, human corneal epithelial, stromal, endothelial and hybrid neuroblastoma cells grew and differentiated on DPC and G-DPC. Thus, GI may achieve effective tissue sterilization without affecting critical properties that are essential for corneal transplant survival. (C) 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
dc.fechaingreso.objetodigital2023-07-18
dc.fuente.origenConveris
dc.identifier.doi10.1016/j.actbio.2019.07.002
dc.identifier.issn1742-7061
dc.identifier.pubmedidPMID: 31284096
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74202
dc.identifier.wosidWOS:000486132900027
dc.information.autorucEscuela de Medicina; Cruzat Corssen, Andrea; 0000-0002-3724-7037; 159755
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final344
dc.pagina.inicio330
dc.revistaActa Biomaterialia
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEffects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts
dc.typeartículo
dc.volumen96
sipa.codpersvinculados159755
sipa.indexWOS
sipa.trazabilidadConveris;20-07-2021
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