Impact of heterologous expression of Escherichia coli UDP-glucose pyrophosphorylase on trehalose and glycogen synthesis in Corynebacterium glutamicum

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dc.contributor.authorPadilla, L
dc.contributor.authorMorbach, S
dc.contributor.authorKramer, R
dc.contributor.authorAgosin, E
dc.date.accessioned2024-01-10T13:13:41Z
dc.date.available2024-01-10T13:13:41Z
dc.date.issued2004
dc.description.abstractTrehalose is a disaccharide with a wide range of applications in the food industry. We recently proposed a strategy for trehalose production based on improved strains of the gram-positive bacterium Corynebacterium glutamicum. This microorganism synthesizes trehalose through two major pathways, OtsBA and TreYZ, by using UDP-glucose and ADP-glucose, respectively, as the glucosyl donors. In this paper we describe improvement of the UDP-glucose supply through heterologous expression in C. glutamicum of the UDP-glucose pyrophosphorylase gene from Escherichia coli, either expressed alone or coexpressed with the E. coli ots genes (galU otsBA synthetic operon). The impact of such expression on trehalose accumulation and excretion, glycogen accumulation, and the growth pattern of new recombinant strains is described. Expression of the galU otsBA synthetic operon resulted in a sixfold increase in the accumulated and excreted trehalose relative to that in a wild-type strain. Surprisingly, single expression of galU also resulted in an increase in the accumulated trehalose. This increase in trehalose synthesis was abolished upon deletion of the TreYZ pathway. These results proved that UDP-glucose has an important role not only in the OtsBA pathway but also in the TreYZ pathway.
dc.fechaingreso.objetodigital2024-05-14
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1128/AEM.70.7.3845-3854.2004
dc.identifier.eissn1098-5336
dc.identifier.issn0099-2240
dc.identifier.pubmedidMEDLINE:15240254
dc.identifier.urihttps://doi.org/10.1128/AEM.70.7.3845-3854.2004
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78329
dc.identifier.wosidWOS:000222758600007
dc.information.autorucIngeniería;Agosin E;S/I;99630
dc.issue.numero7
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final3854
dc.pagina.inicio3845
dc.publisherAMER SOC MICROBIOLOGY
dc.revistaAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
dc.rightsacceso restringido
dc.subjectBIOSYNTHESIS
dc.subjectDNA
dc.subjectOVERPRODUCTION
dc.subjectGROWTH
dc.subjectGENE
dc.subjectPYROPHOSPHATASE
dc.subjectTRANSFORMATION
dc.subjectTRANSCRIPTION
dc.subjectPURIFICATION
dc.subjectACCUMULATION
dc.subject.ods03 Good Health and Well-being
dc.subject.ods12 Responsible Consumption and Production
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa12 Producción y consumo responsable
dc.titleImpact of heterologous expression of Escherichia coli UDP-glucose pyrophosphorylase on trehalose and glycogen synthesis in Corynebacterium glutamicum
dc.typeartículo
dc.volumen70
sipa.codpersvinculados99630
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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