Simvastatin alters fibroblastic cell responses involved in tissue repair

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dc.contributor.authorCaceres, M.
dc.contributor.authorRomero, A.
dc.contributor.authorCopaja, M.
dc.contributor.authorDiaz Araya, G.
dc.contributor.authorMartinez, J.
dc.contributor.authorSmith, P. C.
dc.date.accessioned2024-01-10T14:21:40Z
dc.date.available2024-01-10T14:21:40Z
dc.date.issued2011
dc.description.abstractBackground and Objective: Statins have been used to control hypercholesterolemia. However, these drugs also exert pleiotropic effects that include the modulation of inflammation and cell signaling. The present study has analyzed the effects of simvastatin on several cell responses involved in tissue repair, including cell adhesion, cell migration and invasion, actin cytoskeleton remodeling and cell viability.
dc.description.abstractMaterial and Methods: Primary cultures of gingival fibroblasts were stimulated with simvastatin. Cell adhesion was evaluated using a colorimetric assay. Cell spreading was evaluated microscopically. Cell migration and invasion were assessed using a scratch wound-healing assay and a bicameral cell culture system, respectively. Changes in actin cytoskeleton and focal adhesion assembly were evaluated through immunofluorescence for actin, vinculin and active beta 1 integrin. Rac activation was evaluated by means of a pull-down assay. Cell viability was assessed using a colorimetric assay that determines mitochondrial functionality. Data analysis was performed using the Mann-Whitney U-test.
dc.description.abstractResults: Simvastatin diminished cell adhesion and spreading over a fibronectin matrix. It also altered the closure of scratch wounds induced on cell monolayers and cell invasion through a Transwell system. Simvastatin-treated cells displayed an altered lamellipodia with poorly developed focal adhesion contacts and reduced levels of beta 1 integrin activation. During cell spreading, simvastatin diminished Rac activation.
dc.description.abstractConclusion: The present study shows that simvastatin may alter cell migration by disrupting the cell signaling networks that regulate the actin cytoskeleton dynamics. This mechanism may affect the response of gingival mesenchymal cells during wound healing.
dc.description.funderNational Fund for Science and Technology (FONDECYT) from Chile
dc.fechaingreso.objetodigital2024-05-14
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1111/j.1600-0765.2011.01360.x
dc.identifier.eissn1600-0765
dc.identifier.issn0022-3484
dc.identifier.pubmedidMEDLINE:21395587
dc.identifier.urihttps://doi.org/10.1111/j.1600-0765.2011.01360.x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79748
dc.identifier.wosidWOS:000293018900007
dc.information.autorucMedicina;Smith P;S/I;1006488
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final463
dc.pagina.inicio456
dc.publisherWILEY-BLACKWELL
dc.revistaJOURNAL OF PERIODONTAL RESEARCH
dc.rightsacceso restringido
dc.subjectsimvastatin
dc.subjectwound healing
dc.subjectgingival fibroblast
dc.subjectcell migration
dc.subjectSMOOTH-MUSCLE-CELLS
dc.subjectRHO GTPASES
dc.subjectGROWTH-FACTOR
dc.subjectIN-VIVO
dc.subjectSTATINS
dc.subjectMIGRATION
dc.subjectRAC
dc.subjectMOTILITY
dc.subjectADHESION
dc.subjectKINASE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSimvastatin alters fibroblastic cell responses involved in tissue repair
dc.typeartículo
dc.volumen46
sipa.codpersvinculados1006488
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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