Expression and regulation of scavenger receptor class B type I (SR-BI) in gall bladder epithelium

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dc.contributor.authorMiquel, JF
dc.contributor.authorMoreno, M
dc.contributor.authorAmigo, L
dc.contributor.authorMolina, H
dc.contributor.authorMardones, P
dc.contributor.authorWistuba, II
dc.contributor.authorRigotti, A
dc.date.accessioned2024-01-10T12:38:53Z
dc.date.available2024-01-10T12:38:53Z
dc.date.issued2003
dc.description.abstractBackground and aims: Biliary lipid absorption by the gall bladder mucosa and the cholesterol content of the gall bladder wall appear to play a role in cholesterol gall stone formation. As the scavenger receptor class B type I (SR-BI) regulates cellular cholesterol uptake, we studied its expression in human and murine gall bladders, its regulation by increased biliary lipid content, and its role in gall stone formation.
dc.description.abstractMethods and results: Using immunohistochemistry, SR-BI was found in the apical domain of human gall bladder epithelial cells. Immunoblotting of isolated membranes from gall bladder epithelial cells showed a specific signal for the 82 kDa SR-BI protein. In C57BL/6 mice, SR-BI was also found in the gall bladder epithelium. Using western blot analysis, an inverse relationship was observed between biliary cholesterol concentration and SR-BI expression in murine gall bladder mucosa. By comparing lithogenic diet fed wild-type and SR-BI deficient mice, gall bladder wall cholesterol content and gall stone formation were not found to be dependent on SR-BI expression.
dc.description.abstractConclusions: ( i) SR-BI is expressed in both human and murine gall bladder epithelium; (ii) biliary cholesterol hypersecretion is associated with decreased gall bladder SR-BI expression in mice; and (iii) murine SR-BI is not essential in controlling gall bladder wall cholesterol content and gall stone formation during diet induced cholelithiasis.
dc.fechaingreso.objetodigital2024-05-14
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1136/gut.52.7.1017
dc.identifier.eissn1468-3288
dc.identifier.issn0017-5749
dc.identifier.pubmedidMEDLINE:12801960
dc.identifier.urihttps://doi.org/10.1136/gut.52.7.1017
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77114
dc.identifier.wosidWOS:000183443500019
dc.information.autorucMedicina;Miquel J;S/I;72216
dc.information.autorucMedicina;Rigotti A;S/I;68489
dc.issue.numero7
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1024
dc.pagina.inicio1017
dc.publisherBMJ PUBLISHING GROUP
dc.revistaGUT
dc.rightsacceso restringido
dc.subjectGALLBLADDER MUCOSAL FUNCTION
dc.subjectCHOLESTEROL ABSORPTION
dc.subjectHDL RECEPTOR
dc.subjectDIETARY-CHOLESTEROL
dc.subjectLIPID ABSORPTION
dc.subjectIDENTIFICATION
dc.subjectCRYSTALLIZATION
dc.subjectSOLUBILITY
dc.subjectGALLSTONES
dc.subjectMEMBRANE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleExpression and regulation of scavenger receptor class B type I (SR-BI) in gall bladder epithelium
dc.typeartículo
dc.volumen52
sipa.codpersvinculados72216
sipa.codpersvinculados68489
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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