Biomimetic, Mild Chemical Synthesis of CdTe-GSH Quantum Dots with Improved Biocompatibility

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dc.contributor.authorPerez Donoso, Jose M.
dc.contributor.authorMonras, Juan P.
dc.contributor.authorBravo, Denisse
dc.contributor.authorAguirre, Adam
dc.contributor.authorQuest, Andrew F.
dc.contributor.authorOsorio Roman, Igor O.
dc.contributor.authorAroca, Ricardo F.
dc.contributor.authorChasteen, Thomas G.
dc.contributor.authorVasquez, Claudio C.
dc.date.accessioned2024-01-10T13:46:26Z
dc.date.available2024-01-10T13:46:26Z
dc.date.issued2012
dc.description.abstractMultiple applications of nanotechnology, especially those involving highly fluorescent nanoparticles (NPs) or quantum dots (QDs) have stimulated the research to develop simple, rapid and environmentally friendly protocols for synthesizing NPs exhibiting novel properties and increased biocompatibility. In this study, a simple protocol for the chemical synthesis of glutathione (GSH)-capped CdTe QDs (CdTe-GSH) resembling conditions found in biological systems is described. Using only CdCl2, K2TeO3 and GSH, highly fluorescent QDs were obtained under pH, temperature, buffer and oxygen conditions that allow microorganisms growth. These CdTe-GSH NPs displayed similar size, chemical composition, absorbance and fluorescence spectra and quantum yields as QDs synthesized using more complicated and expensive methods. CdTe QDs were not freely incorporated into eukaryotic cells thus favoring their biocompatibility and potential applications in biomedicine. In addition, NPs entry was facilitated by lipofectamine, resulting in intracellular fluorescence and a slight increase in cell death by necrosis. Toxicity of the as prepared CdTe QDs was lower than that observed with QDs produced by other chemical methods, probably as consequence of decreased levels of Cd+2 and higher amounts of GSH. We present here the simplest, fast and economical method for CdTe QDs synthesis described to date. Also, this biomimetic protocol favors NPs biocompatibility and helps to establish the basis for the development of new, "greener" methods to synthesize cadmium-containing QDs.
dc.description.funderFONDECYT (Fondo Nacional de Investigacion Cientifica y Tecnologica)
dc.description.funderDicyt (Direccion de Investigacion Cientifica y Tecnologica)-USACH (Universidad de Santiago de Chile)
dc.description.funderIFS (International Foundation for Science, Sweden)
dc.description.funderFONDECYT-FONDAP (Fondo de Investigacion Avanzada en Areas Prioritarias)
dc.description.funderRobert A. Welch Foundation
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pone.0030741
dc.identifier.issn1932-6203
dc.identifier.pubmedidMEDLINE:22292028
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0030741
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79161
dc.identifier.wosidWOS:000301570600074
dc.information.autorucQuímica;Osorio I;S/I;207713
dc.issue.numero1
dc.language.isoen
dc.nota.accesoSin adjunto
dc.publisherPUBLIC LIBRARY SCIENCE
dc.revistaPLOS ONE
dc.rightsregistro bibliográfico
dc.subjectSHAPE CONTROL
dc.subjectCELL
dc.subjectNANOPARTICLES
dc.subjectNANOCRYSTALS
dc.subjectGLUTATHIONE
dc.subjectCYTOTOXICITY
dc.subjectTOXICITY
dc.subjectAPOPTOSIS
dc.subjectNECROSIS
dc.subjectCADMIUM
dc.titleBiomimetic, Mild Chemical Synthesis of CdTe-GSH Quantum Dots with Improved Biocompatibility
dc.typeartículo
dc.volumen7
sipa.codpersvinculados207713
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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