Antisense Inhibition of Decorin Expression in Myoblasts Decreases Cell Responsiveness to Transforming Growth Factor B and Accelerates Skeletal Muscle Defferentiation

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dc.catalogadorpva
dc.contributor.authorRiquelme Illanes, Cecilia Angélica
dc.contributor.authorLarraín Correa, Juan Agustín
dc.contributor.authorSchönherr, Elke
dc.contributor.authorHenríquez, Juan Pablo
dc.contributor.authorKresse, Hans
dc.contributor.authorBrandan, Enrique
dc.date.accessioned2017-04-25T19:28:19Z
dc.date.available2017-04-25T19:28:19Z
dc.date.issued2001
dc.description.abstractDecorin is a member of the family of the small leucine-rich proteoglycans. In addition to its function as an extracellular matrix organizer, it has the ability to activate the epidermal growth factor receptor, and it forms complexes with various isoforms of transforming growth factor beta (TGF-beta), Decorin is expressed during skeletal muscle differentiation and is up-regulated in dystrophic muscle. In this study we investigated the role of decorin in TGF-beta -dependent inhibition of myogenesis, To probe the function of decorin during myogenesis, C2C12 myoblasts were stably transfected with a plasmid expressing antisense decorin mRNA. The re; suiting inhibition of decorin expression led to the expression of myogenin, a master transcription factor for muscle differentiation, under growth conditions and accelerated skeletal muscle differentiation as determined by the expression of creatine kinase, In contrast myogenin expression was inhibited by adenovirally induced decorin expression or by adding exogenous decorin, Reduced synthesis of decorin resulted in a 7-fold decreased sensitivity to TGF-beta -mediated inhibition of myogenin expression. In contrast, adenovirally induced decorin expression in wild type cells resulted in a 5-fold increased sensitivity to TGF-beta -mediated inhibition of myogenin expression. Transfection studies with the TGF-beta -dependent promoter of the plasminogen activator inhibitor-1 coupled with luciferase revealed that the transducing receptors for TGF-beta1 and TGF-beta2 were involved in the different responses of wild type and anti-sense decorin myoblasts, These results demonstrate that a reduction of decorin expression or of decorin availability results in a decreased responsiveness to TGF-beta. These findings strongly suggest a new role for decorin during skeletal muscle terminal differentiation by activating TGF-beta -dependent signaling pathways.
dc.fechaingreso.objetodigital2017-04-25
dc.identifier.doi10.1074/jbc.M004602200
dc.identifier.eissn1083-351X
dc.identifier.issn0021-9258
dc.identifier.urihttps://doi.org/10.1074/jbc.M004602200
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/20901
dc.information.autorucFacultad de Ciencias Biológicas; Riquelme Illanes, Cecilia Angélica; S/I; 3268
dc.information.autorucFacultad de Ciencias Biológicas; Larraín Correa, Juan Agustín; S/I; 90468
dc.information.autorucFacultad de Ciencias Biológicas; Henríquez, Juan Pablo; S/I; 5706
dc.information.autorucFacultad de Ciencias Biológicas; Brandan, Enrique; 0000-0002-6820-5059; 52075
dc.issue.numero5
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final3596
dc.pagina.inicio3589
dc.publisherElsevier Inc
dc.revistaJournal of Biological Chemistryes_ES
dc.rightsacceso abierto
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.subject.otherDiferenciación celulares_ES
dc.subject.otherMúsculo esquelético - Patologíaes_ES
dc.subject.otherMúsculo esquelético - Efectos de Drogaes_ES
dc.subject.otherOligonucleótidoses_ES
dc.subject.otherFactor de crecimiento transformante beta1es_ES
dc.titleAntisense Inhibition of Decorin Expression in Myoblasts Decreases Cell Responsiveness to Transforming Growth Factor B and Accelerates Skeletal Muscle Defferentiationes_ES
dc.typeartículo
dc.volumen276
sipa.codpersvinculados90468
sipa.codpersvinculados52075
sipa.codpersvinculados3268
sipa.codpersvinculados5706
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